MetaCyc Pathway: mixed acid fermentation in Rahnella sp. WP5

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Reactions and Genes L-Glutamine (N); ShewMM media; no shaking
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Phosphoenolpyruvate carboxylase (in reverse):
phosphoenolpyruvate + hydrogen carbonate→oxaloacetate + phosphate (EC 4.1.1.31)
EX31_RS14200 -1.3
Pyruvate kinase (in reverse):
phosphoenolpyruvate + ADP + H+→pyruvate + ATP (EC 2.7.1.40)
EX31_RS10640 +0.2
EX31_RS13870 -1.1
D-lactate dehydrogenase (in reverse):
pyruvate + NADH + H+→(R)-lactate + NAD+ (EC 1.1.1.28)
EX31_RS06470 -0.1
EX31_RS23825 -0.0
Malate dehydrogenase (in reverse):
oxaloacetate + NADH + H+→(S)-malate + NAD+ (EC 1.1.1.37; 1.1.1.38)
EX31_RS01150 -0.0
EX31_RS08645 -0.1
EX31_RS18665 +0.2
Formate C-acetyltransferase (in reverse):
pyruvate + coenzyme A→acetyl-CoA + formate (EC 2.3.1.54)
EX31_RS09825 -0.0
EX31_RS09830 -0.2
EX31_RS14675 +0.0
EX31_RS14680 -0.1
EX31_RS18270 -1.0
EX31_RS18470 -0.8
EX31_RS21780 -0.2
Acetaldehyde dehydrogenase (acetylating) (in reverse):
acetyl-CoA + NADH + H+→acetaldehyde + coenzyme A + NAD+ (EC 1.2.1.10)
EX31_RS10075 -1.4
Citrate (Si)-synthase:
acetyl-CoA + oxaloacetate + H2O→citrate + coenzyme A + H+ (EC 2.3.3.1; 2.3.3.16; 2.3.3.3)
EX31_RS23415 -0.3
formate + H+→CO2 + H2
EX31_RS19905 +0.1
EX31_RS19915 +0.1
EX31_RS19950 -0.0
Fumarate hydratase:
(S)-malate→fumarate + H2O (EC 4.2.1.2)
EX31_RS01160 -0.2
EX31_RS06965 +0.1
Phosphate acetyltransferase:
acetyl-CoA + phosphate→acetyl phosphate + coenzyme A (EC 2.3.1.8)
EX31_RS19485 -1.5
Acetate kinase (in reverse):
acetyl phosphate + ADP→acetate + ATP (EC 2.7.2.1; 2.7.2.15)
EX31_RS19480 -1.1
EX31_RS24735 -0.4
Aconitate hydratase:
citrate→cis-aconitate + H2O (EC 4.2.1.3)
EX31_RS03400 -0.1
EX31_RS09885 -0.5
Alcohol dehydrogenase (in reverse):
acetaldehyde + NADH + H+→ethanol + NAD+ (EC 1.1.1.1)
EX31_RS00895 +0.1
EX31_RS08040 +0.1
EX31_RS10075 -1.4
EX31_RS10950 +0.3
EX31_RS15570 -0.0
EX31_RS16670 -0.6
EX31_RS18715 -0.1
Succinate dehydrogenase (ubiquinone):
fumarate[in] + a menaquinol→succinate[in] + a menaquinone (EC 1.3.5.1)
EX31_RS08900 -0.2
EX31_RS08905 -0.4
EX31_RS08910 -0.2
EX31_RS08915 -0.3
Aconitate hydratase:
cis-aconitate + H2O→D-threo-isocitrate (EC 4.2.1.3)
EX31_RS03400 -0.1
EX31_RS09885 -0.5
Isocitrate dehydrogenase (NADP(+)):
D-threo-isocitrate + NADP+→CO2 + 2-oxoglutarate + NADPH (EC 1.1.1.42)
EX31_RS24590 N.D.

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Candidate genes for each reaction are identified from best hits to MetaCyc; by matching EC numbers (which are assigned by TIGRFam, SEED, or best hits to KEGG); or by matching SEED roles to KEGG reactions to MetaCyc reactions. See the "Protein" tab of each gene for more information