MetaCyc Pathway: O-antigen building blocks biosynthesis (E. coli) in Pseudomonas fluorescens FW300-N2E3

Reactions and Genes	D-Fructose (C)
UDP-galactopyranose mutase: UDP-α-D-galactose→UDP-α-D-galactofuranose (EC 5.4.99.9)
No genes
Glucose-6-phosphate isomerase: α-D-glucose 6-phosphate→β-D-fructofuranose 6-phosphate (EC 5.3.1.9)
AO353_14370	-3.0
Phosphoglucomutase (in reverse): D-glucopyranose 6-phosphate→α-D-glucopyranose 1-phosphate (EC 5.4.2.2)
AO353_05595	N.D.
AO353_09235	+0.6
AO353_24665	+2.1
Glucose-1-phosphate thymidylyltransferase: α-D-glucopyranose 1-phosphate + H+ + dTTP→dTDP-α-D-glucose + diphosphate (EC 2.7.7.24)
AO353_07290	+0.4
AO353_11880	N.D.
Glutamine--fructose-6-phosphate transaminase (isomerizing): β-D-fructofuranose 6-phosphate + L-glutamine→D-glucosamine 6-phosphate + L-glutamate (EC 2.6.1.16)
AO353_10370	N.D.
Phosphoglucosamine mutase: D-glucosamine 6-phosphate→α-D-glucosamine 1-phosphate (EC 5.4.2.10)
AO353_05595	N.D.
dTDP-glucose 4,6-dehydratase: dTDP-α-D-glucose→dTDP-4-dehydro-6-deoxy-α-D-glucopyranose + H2O (EC 4.2.1.46)
AO353_11885	N.D.
Glucosamine-1-phosphate N-acetyltransferase: α-D-glucosamine 1-phosphate + acetyl-CoA→N-acetyl-α-D-glucosamine 1-phosphate + coenzyme A + H+ (EC 2.3.1.157)
AO353_10380	N.D.
dTDP-4-dehydrorhamnose 3,5-epimerase: dTDP-4-dehydro-6-deoxy-α-D-glucopyranose→dTDP-4-dehydro-β-L-rhamnose (EC 5.1.3.13)
AO353_11875	N.D.
dTDP-4-dehydrorhamnose reductase (in reverse): dTDP-4-dehydro-β-L-rhamnose + NADPH + H+→dTDP-β-L-rhamnose + NADP+ (EC 1.1.1.133)
AO353_02030	-0.5
AO353_06775	-0.4
AO353_11870	N.D.
UDP-N-acetylglucosamine diphosphorylase: N-acetyl-α-D-glucosamine 1-phosphate + H+ + UTP→UDP-N-acetyl-α-D-glucosamine + diphosphate (EC 2.7.7.23)
AO353_10380	N.D.

Candidate genes for each reaction are identified from best hits to MetaCyc; by matching EC numbers (which are assigned by TIGRFam, SEED, or best hits to KEGG); or by matching SEED roles to KEGG reactions to MetaCyc reactions. See the "Protein" tab of each gene for more information