MetaCyc Pathway: partial TCA cycle (obligate autotrophs) in Pseudomonas fluorescens FW300-N2E3

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Reactions and Genes propionate (C)
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Phosphoenolpyruvate carboxylase (in reverse):
phosphoenolpyruvate + hydrogen carbonate→oxaloacetate + phosphate (EC 4.1.1.31)
AO353_16310 -2.1
Citrate (Si)-synthase:
oxaloacetate + acetyl-CoA + H2O→citrate + coenzyme A + H+ (EC 2.3.3.1; 2.3.3.16; 2.3.3.3)
AO353_01860 N.D.
oxaloacetate[in] + an electron-transfer quinol→(S)-malate[in] + an electron-transfer quinone (EC 1.1.5.4)
AO353_14940 -3.7
AO353_28515 +0.4
Aconitate hydratase:
citrate→cis-aconitate + H2O (EC 4.2.1.3)
AO353_00565 -0.0
AO353_00890 -2.9
AO353_21605 N.D.
Fumarate hydratase:
(S)-malate→fumarate + H2O (EC 4.2.1.2)
AO353_02150 +0.1
AO353_03795 -1.5
Aconitate hydratase:
cis-aconitate + H2O→D-threo-isocitrate (EC 4.2.1.3)
AO353_00565 -0.0
AO353_00890 -2.9
AO353_21605 N.D.
Succinate dehydrogenase (ubiquinone) (in reverse):
fumarate[in] + an electron-transfer quinol→succinate[in] + an electron-transfer quinone (EC 1.3.5.1)
AO353_01840 N.D.
AO353_01845 N.D.
AO353_01850 N.D.
Isocitrate dehydrogenase (NADP(+)):
D-threo-isocitrate + NADP+→2-oxoglutarate + CO2 + NADPH (EC 1.1.1.42)
AO353_27690 +0.6
AO353_27695 -0.5

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Candidate genes for each reaction are identified from best hits to MetaCyc; by matching EC numbers (which are assigned by TIGRFam, SEED, or best hits to KEGG); or by matching SEED roles to KEGG reactions to MetaCyc reactions. See the "Protein" tab of each gene for more information