MetaCyc Pathway: TCA cycle I (prokaryotic) in Azospirillum brasilense Sp245

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Reactions and Genes D-Cycloserine 0.125 mg/ml
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2-oxoglutarate + coenzyme A + NAD+→succinyl-CoA + CO2 + NADH (EC 1.2.1.105)
AZOBR_RS14390 N.D.
AZOBR_RS22245 N.D.
AZOBR_RS22250 N.D.
AZOBR_RS25395 -0.6
Succinate--CoA ligase (ADP-forming) (in reverse):
succinyl-CoA + ADP + phosphate→succinate + ATP + coenzyme A (EC 6.2.1.5)
AZOBR_RS14065 -0.1
AZOBR_RS14070 +0.1
AZOBR_RS32480 +0.4
AZOBR_RS32485 -0.2
Succinate dehydrogenase (ubiquinone):
succinate[in] + an electron-transfer quinone→fumarate[in] + an electron-transfer quinol (EC 1.3.5.1)
No genes
Fumarate hydratase (in reverse):
fumarate + H2O→(S)-malate (EC 4.2.1.2)
AZOBR_RS06120 +1.4
AZOBR_RS10315 -0.3
Malate dehydrogenase:
(S)-malate + NAD+→oxaloacetate + NADH + H+ (EC 1.1.1.37; 1.1.1.38)
AZOBR_RS14060 N.D.
AZOBR_RS16020 -0.3
(S)-malate[in] + an electron-transfer quinone→oxaloacetate[in] + an electron-transfer quinol (EC 1.1.5.4)
No genes
Citrate (Si)-synthase:
oxaloacetate + acetyl-CoA + H2O→citrate + coenzyme A + H+ (EC 2.3.3.1; 2.3.3.16; 2.3.3.3)
AZOBR_RS25925 -0.1
Aconitate hydratase:
citrate→cis-aconitate + H2O (EC 4.2.1.3)
AZOBR_RS02985 +1.1
AZOBR_RS17090 N.D.
Aconitate hydratase:
cis-aconitate + H2O→D-threo-isocitrate (EC 4.2.1.3)
AZOBR_RS02985 +1.1
AZOBR_RS17090 N.D.
Isocitrate dehydrogenase (NADP(+)):
D-threo-isocitrate + NADP+→2-oxoglutarate + CO2 + NADPH (EC 1.1.1.42)
AZOBR_RS10995 N.D.
AZOBR_RS11025 -0.3

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Candidate genes for each reaction are identified from best hits to MetaCyc; by matching EC numbers (which are assigned by TIGRFam, SEED, or best hits to KEGG); or by matching SEED roles to KEGG reactions to MetaCyc reactions. See the "Protein" tab of each gene for more information