MetaCyc Pathway: 3-methyl-branched fatty acid α-oxidation in Pseudomonas fluorescens FW300-N2E3

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Reactions and Genes acetate (C)
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Long-chain-fatty-acid--CoA ligase:
a 3-methyl-branched 2,3,4-saturated fatty acid + ATP + coenzyme A→a 3-methyl-branched 2,3,4-saturated fatty acyl-CoA + AMP + diphosphate
(EC 6.2.1.3)
AO353_02215 -0.2
AO353_03310 +1.7
AO353_03315 -0.1
AO353_20355 -0.0
a 3-methyl-branched 2,3,4-saturated fatty acyl-CoA + 2-oxoglutarate + dioxygen→a 2-hydroxy-3-methyl-branched 2,3,4-saturated fatty acyl-CoA + CO2 + succinate
No genes
a 2-hydroxy-3-methyl-branched 2,3,4-saturated fatty acyl-CoA→a 2-methyl branched 2,3,4-saturated fatty aldehyde + formyl-CoA (EC 4.1.2.63)
No genes
Formyl-CoA hydrolase:
formyl-CoA + H2O→formate + coenzyme A + H+
(EC 3.1.2.10)
No genes
Aldehyde dehydrogenase (NAD(+)):
a 2-methyl branched 2,3,4-saturated fatty aldehyde + NAD+ + H2O→a 2-methyl branched 2,3,4-saturated fatty acid + NADH + 2 H+
(EC 1.2.1.3)
AO353_03860 -0.3
AO353_05300 -0.0
AO353_06560 +0.0
AO353_08290 +0.3
AO353_09195 -0.4
AO353_13330 -0.3
AO353_15035 -0.2
AO353_19510 -0.0
AO353_22390 -0.3
AO353_23135 +0.0
AO353_24855 +0.1
AO353_24905 -0.3
AO353_28290 +0.1
Long-chain-fatty-acid--CoA ligase:
a 2-methyl branched 2,3,4-saturated fatty acid + ATP + coenzyme A→a 2-methyl branched 2,3,4-saturated fatty acyl-CoA + AMP + diphosphate
(EC 6.2.1.3)
AO353_02215 -0.2
AO353_03310 +1.7
AO353_03315 -0.1
AO353_20355 -0.0

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Candidate genes for each reaction are identified from best hits to MetaCyc; by matching EC numbers (which are assigned by TIGRFam, SEED, or best hits to KEGG); or by matching SEED roles to KEGG reactions to MetaCyc reactions. See the "Protein" tab of each gene for more information