MetaCyc Pathway: palmitate biosynthesis III in Pseudomonas fluorescens FW300-N2E3

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Reactions and Genes acetate (C)
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3-oxoacyl-[acyl-carrier-protein] reductase (in reverse):
an acetoacetyl-[acp] + NADPH + H+→a (3R)-3-hydroxybutanoyl-[acp] + NADP+ (EC 1.1.1.100)
AO353_00945 -0.0
AO353_01365 N.D.
AO353_04045 +0.1
AO353_11955 -0.1
AO353_12610 -0.3
AO353_13875 -0.2
AO353_22325 -0.0
AO353_23150 N.D.
AO353_23170 +0.4
AO353_24520 +0.5
AO353_28590 -0.1
3-hydroxyoctanoyl-[acyl-carrier-protein] dehydratase:
a (3R)-3-hydroxybutanoyl-[acp]→a (2E)-but-2-enoyl-[acp] + H2O (EC 4.2.1.59)
AO353_01755 N.D.
AO353_16515 N.D.
Enoyl-[acyl-carrier-protein] reductase (NADPH, B-specific) (in reverse):
a (2E)-but-2-enoyl-[acp] + NADPH + H+→a butanoyl-[acp] + NADP+ (EC 1.3.1.10; 1.3.1.104; 1.3.1.39)
No genes
Beta-ketoacyl-acyl-carrier-protein synthase II:
a butanoyl-[acp] + a malonyl-[acp] + H+→a 3-oxohexanoyl-[acp] + a soluble [acyl-carrier protein] + CO2 (EC 2.3.1.179; 2.3.1.41)
AO353_01355 -0.8
AO353_01760 N.D.
AO353_10365 +0.3
AO353_12605 +0.0
AO353_12620 -0.3
AO353_22590 -0.3
3-oxoacyl-[acyl-carrier-protein] reductase (in reverse):
a 3-oxohexanoyl-[acp] + NADPH + H+→a (3R)-3-hydroxyhexanoyl-[acp] + NADP+ (EC 1.1.1.100)
AO353_00945 -0.0
AO353_01365 N.D.
AO353_04045 +0.1
AO353_11955 -0.1
AO353_12610 -0.3
AO353_13875 -0.2
AO353_22325 -0.0
AO353_23150 N.D.
AO353_23170 +0.4
AO353_24520 +0.5
AO353_28590 -0.1
3-hydroxyoctanoyl-[acyl-carrier-protein] dehydratase:
a (3R)-3-hydroxyhexanoyl-[acp]→a (2E)-hex-2-enoyl-[acp] + H2O (EC 4.2.1.59)
AO353_01755 N.D.
AO353_16515 N.D.
Enoyl-[acyl-carrier-protein] reductase (NADPH, B-specific) (in reverse):
a (2E)-hex-2-enoyl-[acp] + NADPH + H+→a hexanoyl-[acp] + NADP+ (EC 1.3.1.10; 1.3.1.104; 1.3.1.39)
No genes
Beta-ketoacyl-acyl-carrier-protein synthase II:
a hexanoyl-[acp] + a malonyl-[acp] + H+→a 3-oxooctanoyl-[acp] + a soluble [acyl-carrier protein] + CO2 (EC 2.3.1.179; 2.3.1.41)
AO353_01355 -0.8
AO353_01760 N.D.
AO353_10365 +0.3
AO353_12605 +0.0
AO353_12620 -0.3
AO353_22590 -0.3
3-oxoacyl-[acyl-carrier-protein] reductase (in reverse):
a 3-oxooctanoyl-[acp] + NADPH + H+→a (3R)-3-hydroxyoctanoyl-[acp] + NADP+ (EC 1.1.1.100)
AO353_00945 -0.0
AO353_01365 N.D.
AO353_04045 +0.1
AO353_11955 -0.1
AO353_12610 -0.3
AO353_13875 -0.2
AO353_22325 -0.0
AO353_23150 N.D.
AO353_23170 +0.4
AO353_24520 +0.5
AO353_28590 -0.1
3-hydroxyoctanoyl-[acyl-carrier-protein] dehydratase:
a (3R)-3-hydroxyoctanoyl-[acp]→a (2E)-oct-2-enoyl-[acp] + H2O (EC 4.2.1.59)
AO353_01755 N.D.
AO353_16515 N.D.
Enoyl-[acyl-carrier-protein] reductase (NADPH, B-specific) (in reverse):
a (2E)-oct-2-enoyl-[acp] + NADPH + H+→an octanoyl-[acp] + NADP+ (EC 1.3.1.10; 1.3.1.104; 1.3.1.39)
No genes
Beta-ketoacyl-acyl-carrier-protein synthase II:
an octanoyl-[acp] + a malonyl-[acp] + H+→a 3-oxodecanoyl-[acp] + a soluble [acyl-carrier protein] + CO2 (EC 2.3.1.179; 2.3.1.41)
AO353_01355 -0.8
AO353_01760 N.D.
AO353_10365 +0.3
AO353_12605 +0.0
AO353_12620 -0.3
AO353_22590 -0.3
3-oxoacyl-[acyl-carrier-protein] reductase (in reverse):
a 3-oxodecanoyl-[acp] + NADPH + H+→a (3R)-3-hydroxydecanoyl-[acp] + NADP+ (EC 1.1.1.100)
AO353_00945 -0.0
AO353_01365 N.D.
AO353_04045 +0.1
AO353_11955 -0.1
AO353_12610 -0.3
AO353_13875 -0.2
AO353_22325 -0.0
AO353_23150 N.D.
AO353_23170 +0.4
AO353_24520 +0.5
AO353_28590 -0.1
3-hydroxyoctanoyl-[acyl-carrier-protein] dehydratase:
a (3R)-3-hydroxydecanoyl-[acp]→a (2E)-dec-2-enoyl-[acp] + H2O (EC 4.2.1.59)
AO353_01755 N.D.
AO353_16515 N.D.
Enoyl-[acyl-carrier-protein] reductase (NADPH, B-specific) (in reverse):
a (2E)-dec-2-enoyl-[acp] + NADPH + H+→a decanoyl-[acp] + NADP+ (EC 1.3.1.10; 1.3.1.104; 1.3.1.39)
No genes
Beta-ketoacyl-acyl-carrier-protein synthase II:
a decanoyl-[acp] + a malonyl-[acp] + H+→a 3-oxododecanoyl-[acp] + a soluble [acyl-carrier protein] + CO2 (EC 2.3.1.179; 2.3.1.41)
AO353_01355 -0.8
AO353_01760 N.D.
AO353_10365 +0.3
AO353_12605 +0.0
AO353_12620 -0.3
AO353_22590 -0.3
3-oxoacyl-[acyl-carrier-protein] reductase (in reverse):
a 3-oxododecanoyl-[acp] + NADPH + H+→a (3R)-3-hydroxydodecanoyl-[acp] + NADP+ (EC 1.1.1.100)
AO353_00945 -0.0
AO353_01365 N.D.
AO353_04045 +0.1
AO353_11955 -0.1
AO353_12610 -0.3
AO353_13875 -0.2
AO353_22325 -0.0
AO353_23150 N.D.
AO353_23170 +0.4
AO353_24520 +0.5
AO353_28590 -0.1
3-hydroxyoctanoyl-[acyl-carrier-protein] dehydratase:
a (3R)-3-hydroxydodecanoyl-[acp]→a (2E)-dodec-2-enoyl-[acp] + H2O (EC 4.2.1.59)
AO353_01755 N.D.
AO353_16515 N.D.
Enoyl-[acyl-carrier-protein] reductase (NADPH, B-specific) (in reverse):
a (2E)-dodec-2-enoyl-[acp] + NADPH + H+→a dodecanoyl-[acp] + NADP+ (EC 1.3.1.10; 1.3.1.104; 1.3.1.39)
No genes
Beta-ketoacyl-acyl-carrier-protein synthase II:
a dodecanoyl-[acp] + a malonyl-[acp] + H+→a 3-oxotetradecanoyl-[acp] + a soluble [acyl-carrier protein] + CO2 (EC 2.3.1.179; 2.3.1.41)
AO353_01355 -0.8
AO353_01760 N.D.
AO353_10365 +0.3
AO353_12605 +0.0
AO353_12620 -0.3
AO353_22590 -0.3
3-oxoacyl-[acyl-carrier-protein] reductase (in reverse):
a 3-oxotetradecanoyl-[acp] + NADPH + H+→a (3R)-3-hydroxytetradecanoyl-[acp] + NADP+ (EC 1.1.1.100)
AO353_00945 -0.0
AO353_01365 N.D.
AO353_04045 +0.1
AO353_11955 -0.1
AO353_12610 -0.3
AO353_13875 -0.2
AO353_22325 -0.0
AO353_23150 N.D.
AO353_23170 +0.4
AO353_24520 +0.5
AO353_28590 -0.1
3-hydroxyoctanoyl-[acyl-carrier-protein] dehydratase:
a (3R)-3-hydroxytetradecanoyl-[acp]→a (2E)-tetradec-2-enoyl-[acp] + H2O (EC 4.2.1.59)
AO353_01755 N.D.
AO353_16515 N.D.
Enoyl-[acyl-carrier-protein] reductase (NADPH, B-specific) (in reverse):
a (2E)-tetradec-2-enoyl-[acp] + NADPH + H+→a myristoyl-[acp] + NADP+ (EC 1.3.1.10; 1.3.1.104; 1.3.1.39)
No genes
Beta-ketoacyl-acyl-carrier-protein synthase II:
a myristoyl-[acp] + a malonyl-[acp] + H+→a 3-oxohexadecanoyl-[acp] + a soluble [acyl-carrier protein] + CO2 (EC 2.3.1.179; 2.3.1.41)
AO353_01355 -0.8
AO353_01760 N.D.
AO353_10365 +0.3
AO353_12605 +0.0
AO353_12620 -0.3
AO353_22590 -0.3
3-oxoacyl-[acyl-carrier-protein] reductase (in reverse):
a 3-oxohexadecanoyl-[acp] + NADPH + H+→a (3R)-3-hydroxyhexadecanoyl-[acp] + NADP+ (EC 1.1.1.100)
AO353_00945 -0.0
AO353_01365 N.D.
AO353_04045 +0.1
AO353_11955 -0.1
AO353_12610 -0.3
AO353_13875 -0.2
AO353_22325 -0.0
AO353_23150 N.D.
AO353_23170 +0.4
AO353_24520 +0.5
AO353_28590 -0.1
3-hydroxyoctanoyl-[acyl-carrier-protein] dehydratase:
a (3R)-3-hydroxyhexadecanoyl-[acp]→a (2E)-hexadec-2-enoyl-[acp] + H2O (EC 4.2.1.59)
AO353_01755 N.D.
AO353_16515 N.D.
Enoyl-[acyl-carrier-protein] reductase (NADPH, B-specific) (in reverse):
a (2E)-hexadec-2-enoyl-[acp] + NADPH + H+→a palmitoyl-[acp] + NADP+ (EC 1.3.1.10; 1.3.1.104; 1.3.1.39)
No genes
Oleoyl-[acyl-carrier-protein] hydrolase:
a palmitoyl-[acp] + H2O→palmitate + a soluble [acyl-carrier protein] + H+ (EC 3.1.2.14)
No genes
Long-chain-fatty-acid--CoA ligase:
palmitate + ATP + coenzyme A→palmitoyl-CoA + AMP + diphosphate (EC 6.2.1.3)
AO353_02215 -0.2
AO353_03310 +1.7
AO353_03315 -0.1
AO353_20355 -0.0

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Candidate genes for each reaction are identified from best hits to MetaCyc; by matching EC numbers (which are assigned by TIGRFam, SEED, or best hits to KEGG); or by matching SEED roles to KEGG reactions to MetaCyc reactions. See the "Protein" tab of each gene for more information