MetaCyc Pathway: fatty acid salvage in Pseudomonas fluorescens FW300-N2E3

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Reactions and Genes acetate (C)
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Butyrate--CoA ligase:
decanoate + ATP + coenzyme A→decanoyl-CoA + AMP + diphosphate (EC 6.2.1.2; 6.2.1.3)
AO353_02215 -0.2
AO353_03310 +1.7
AO353_03315 -0.1
AO353_20355 -0.0
decanoyl-CoA + an oxidized electron-transfer flavoprotein + H+→(2E)-dec-2-enoyl-CoA + a reduced electron-transfer flavoprotein (EC 1.3.8.7)
AO353_01500 -0.1
AO353_06340 +0.1
AO353_07485 -0.1
AO353_07495 +0.4
AO353_12805 -0.2
AO353_21065 +0.1
Long-chain-enoyl-CoA hydratase (in reverse):
(2E)-dec-2-enoyl-CoA + H2O→(S)-3-hydroxydecanoyl-CoA (EC 4.2.1.74)
AO353_00510 +0.3
AO353_03780 -0.2
AO353_17555 -0.1
AO353_19125 N.D.
AO353_23055 +0.5
AO353_24830 -1.6
3-hydroxyacyl-CoA dehydrogenase:
(S)-3-hydroxydecanoyl-CoA + NAD+→3-oxodecanoyl-CoA + NADH + H+ (EC 1.1.1.35)
AO353_00510 +0.3
AO353_01155 +0.2
Acetyl-CoA C-acyltransferase (in reverse):
3-oxodecanoyl-CoA + coenzyme A→octanoyl-CoA + acetyl-CoA (EC 2.3.1.16)
AO353_00505 +0.5
AO353_01065 +3.5
AO353_01160 -0.2
AO353_05785 +0.0
AO353_13880 +0.1
AO353_17205 -0.4
AO353_25685 +0.4
AO353_27530 +0.4
AO353_27945 +0.0
octanoyl-CoA + a malonyl-[acp] + H+→a 3-oxodecanoyl-[acp] + CO2 + coenzyme A (EC 2.3.1.207)
AO353_18230 +0.1

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Candidate genes for each reaction are identified from best hits to MetaCyc; by matching EC numbers (which are assigned by TIGRFam, SEED, or best hits to KEGG); or by matching SEED roles to KEGG reactions to MetaCyc reactions. See the "Protein" tab of each gene for more information