MetaCyc Pathway: queuosine biosynthesis I (de novo) in Pseudomonas fluorescens FW300-N2E3

Reactions and Genes	acetate (C)
PreQ(1) synthase (in reverse): preQ0 + 2 NADPH + 3 H+→preQ1 + 2 NADP+ (EC 1.7.1.13)
AO353_00345	-1.5
tRNA-guanine transglycosylase: preQ1 + a guanine34 in tRNA→a 7-aminomethyl-7-deazaguanosine34 in tRNA + guanine (EC 2.4.2.29)
AO353_15790	N.D.
a 7-aminomethyl-7-deazaguanosine34 in tRNA + S-adenosyl-L-methionine→an epoxyqueuosine34 in tRNA + adenine + L-methionine + H+ (EC 2.4.99.17)
AO353_15785	-2.0
an epoxyqueuosine34 in tRNA + a reduced two electron carrier→a queuosine34 in tRNA + an oxidized electron carrier + H2O (EC 1.17.99.6)
AO353_13115	-0.0

Candidate genes for each reaction are identified from best hits to MetaCyc; by matching EC numbers (which are assigned by TIGRFam, SEED, or best hits to KEGG); or by matching SEED roles to KEGG reactions to MetaCyc reactions. See the "Protein" tab of each gene for more information