MetaCyc Pathway: 3-hydroxypropanoate/4-hydroxybutanate cycle in Pseudomonas fluorescens FW300-N2E3

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Reactions and Genes acetate (C)
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Carbonate dehydratase (in reverse):
CO2 + H2O→hydrogen carbonate + H+ (EC 4.2.1.1)
AO353_10145 +1.1
AO353_10815 -3.0
AO353_14595 -1.9
AO353_25445 +0.1
AO353_27095 +0.1
Biotin carboxylase:
hydrogen carbonate + ATP + a [biotin carboxyl-carrier-protein dimer]-N6-biotinyl-L-lysine→a [carboxyl-carrier protein dimer]-N6-carboxybiotinyl-L-lysine + ADP + H+ + phosphate (EC 6.3.4.14)
AO353_07105 +0.2
AO353_13675 N.D.
AO353_13680 N.D.
AO353_28810 +0.5
a [carboxyl-carrier protein dimer]-N6-carboxybiotinyl-L-lysine + acetyl-CoA→malonyl-CoA + a [biotin carboxyl-carrier-protein dimer]-N6-biotinyl-L-lysine (EC 2.1.3.15)
AO353_16540 N.D.
AO353_20670 N.D.
malonyl-CoA + NADPH + H+→3-oxopropanoate + coenzyme A + NADP+ (EC 1.2.1.75)
No genes
3-oxopropanoate + NADPH + H+→3-hydroxypropanoate + NADP+ (EC 1.1.1.298)
No genes
3-hydroxypropanoate + ATP + coenzyme A→3-hydroxypropanoyl-CoA + AMP + diphosphate (EC 6.2.1.36)
No genes
3-hydroxypropanoyl-CoA→acryloyl-CoA + H2O (EC 4.2.1.116)
AO353_25675 N.D.
acryloyl-CoA + NADPH + H+→propanoyl-CoA + NADP+ (EC 1.3.1.84)
No genes
Propionyl-CoA carboxylase:
hydrogen carbonate + propanoyl-CoA + ATP→(S)-methylmalonyl-CoA + ADP + H+ + phosphate (EC 6.4.1.3)
AO353_16540 N.D.
AO353_20670 N.D.
Methylmalonyl-CoA epimerase (in reverse):
(S)-methylmalonyl-CoA→(R)-methylmalonyl-CoA (EC 5.1.99.1)
No genes
Methylmalonyl-CoA mutase:
(R)-methylmalonyl-CoA→succinyl-CoA (EC 5.4.99.2)
No genes
succinyl-CoA + NADPH + H+→succinate semialdehyde + coenzyme A + NADP+ (EC 1.2.1.76)
No genes
succinate semialdehyde + NADPH + H+→4-hydroxybutanoate + NADP+ (EC 1.1.1.co)
No genes
4-hydroxybutanoate + ATP + coenzyme A→4-hydroxybutanoyl-CoA + AMP + diphosphate (EC 6.2.1.40)
No genes
4-hydroxybutanoyl-CoA→crotonyl-CoA + H2O (EC 4.2.1.120)
No genes
crotonyl-CoA + H2O→(S)-3-hydroxybutanoyl-CoA (EC 4.2.1.150)
AO353_00510 +0.3
AO353_03780 -0.2
AO353_17555 -0.1
AO353_19125 N.D.
AO353_23055 +0.5
AO353_24830 -1.6
3-hydroxyacyl-CoA dehydrogenase:
(S)-3-hydroxybutanoyl-CoA + NAD+→acetoacetyl-CoA + NADH + H+ (EC 1.1.1.35)
AO353_00510 +0.3
AO353_01155 +0.2
AO353_27535 -0.5
Acetyl-CoA C-acyltransferase (in reverse):
acetoacetyl-CoA + coenzyme A→2 acetyl-CoA (EC 2.3.1.16; 2.3.1.9)
AO353_00505 +0.5
AO353_01065 +3.5
AO353_01160 -0.2
AO353_05785 +0.0
AO353_13880 +0.1
AO353_17205 -0.4
AO353_25685 +0.4
AO353_27530 +0.4
AO353_27945 +0.0

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Candidate genes for each reaction are identified from best hits to MetaCyc; by matching EC numbers (which are assigned by TIGRFam, SEED, or best hits to KEGG); or by matching SEED roles to KEGG reactions to MetaCyc reactions. See the "Protein" tab of each gene for more information