MetaCyc Pathway: glyoxylate assimilation in Pseudomonas fluorescens FW300-N2E3

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Reactions and Genes acetate (C)
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Carbonate dehydratase (in reverse):
CO2 + H2O→hydrogen carbonate + H+ (EC 4.2.1.1)
AO353_10145 +1.1
AO353_10815 -3.0
AO353_14595 -1.9
AO353_25445 +0.1
AO353_27095 +0.1
Biotin carboxylase:
hydrogen carbonate + ATP + a [biotin carboxyl-carrier-protein dimer]-N6-biotinyl-L-lysine→a [carboxyl-carrier protein dimer]-N6-carboxybiotinyl-L-lysine + ADP + H+ + phosphate (EC 6.3.4.14)
AO353_07105 +0.2
AO353_13675 N.D.
AO353_13680 N.D.
AO353_28810 +0.5
a [carboxyl-carrier protein dimer]-N6-carboxybiotinyl-L-lysine + acetyl-CoA→malonyl-CoA + a [biotin carboxyl-carrier-protein dimer]-N6-biotinyl-L-lysine (EC 2.1.3.15)
AO353_16540 N.D.
AO353_20670 N.D.
malonyl-CoA + NADPH + H+→3-oxopropanoate + coenzyme A + NADP+ (EC 1.2.1.75)
No genes
3-oxopropanoate + NADPH + H+→3-hydroxypropanoate + NADP+ (EC 1.1.1.298)
No genes
3-hydroxypropanoate + ATP + coenzyme A→3-hydroxypropanoyl-CoA + AMP + diphosphate (EC 6.2.1.36)
No genes
3-hydroxypropanoyl-CoA→acryloyl-CoA + H2O (EC 4.2.1.116)
AO353_25675 N.D.
acryloyl-CoA + NADPH + H+→propanoyl-CoA + NADP+ (EC 1.3.1.84)
No genes
Malyl-CoA lyase (in reverse):
propanoyl-CoA + glyoxylate→(2R,3S)-β-methylmalyl-CoA (EC 4.1.3.24)
No genes
(2R,3S)-β-methylmalyl-CoA→2-methylfumaryl-CoA + H2O (EC 4.2.1.148)
No genes
2-methylfumaryl-CoA→3-methylfumaryl-CoA (EC 5.4.1.3)
No genes
3-methylfumaryl-CoA + H2O→(3S)-citramalyl-CoA (EC 4.2.1.153)
No genes
Citramalyl-CoA lyase:
(3S)-citramalyl-CoA→acetyl-CoA + pyruvate (EC 4.1.3.25)
No genes

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Candidate genes for each reaction are identified from best hits to MetaCyc; by matching EC numbers (which are assigned by TIGRFam, SEED, or best hits to KEGG); or by matching SEED roles to KEGG reactions to MetaCyc reactions. See the "Protein" tab of each gene for more information