MetaCyc Pathway: glutaminyl-tRNAgln biosynthesis via transamidation in Rahnella sp. WP5

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Reactions and Genes L-Glutamine (N); RCH2 media; shaking
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Glutamate synthase (NADPH):
L-glutamine + H2O→ammonia + L-glutamate + H+ (EC 1.4.1.13; 1.4.1.14; 1.4.7.1; 2.6.1.85; 3.5.1.2; 3.5.1.38; 4.1.3.27; 4.3.2.10; 4.3.3.6; 6.3.4.2; 6.3.5.13; 6.3.5.2; 6.3.5.4; 6.3.5.5; 6.3.5.6; 6.3.5.7)
EX31_RS01830 +0.1
EX31_RS02720 -1.7
EX31_RS03830 -4.8
EX31_RS03835 -4.9
EX31_RS04635 +0.2
EX31_RS07260 +0.2
EX31_RS07945 N.D.
EX31_RS08365 -0.2
EX31_RS09955 -4.0
EX31_RS09960 -3.4
EX31_RS12510 -4.6
EX31_RS12515 -3.9
EX31_RS13390 -0.1
EX31_RS17520 +0.0
EX31_RS20445 N.D.
EX31_RS21080 -0.3
EX31_RS21140 +0.0
EX31_RS23245 -5.1
EX31_RS24725 -0.2
Glutamate--tRNA(Gln) ligase:
L-glutamate + ATP + a tRNAGln→an L-glutamyl-[tRNAGln] + AMP + diphosphate (EC 6.1.1.24)
No genes
Glutaminyl-tRNA synthase (glutamine-hydrolyzing):
an L-glutamyl-[tRNAGln] + ATP→a 5-phosphooxy-L-glutamyl-[tRNAGln] + ADP (EC 6.3.5.7)
EX31_RS21140 +0.0
Glutaminyl-tRNA synthase (glutamine-hydrolyzing):
a 5-phosphooxy-L-glutamyl-[tRNAGln] + ammonia→an L-glutaminyl-[tRNAGln] + phosphate (EC 6.3.5.7)
EX31_RS21140 +0.0

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Candidate genes for each reaction are identified from best hits to MetaCyc; by matching EC numbers (which are assigned by TIGRFam, SEED, or best hits to KEGG); or by matching SEED roles to KEGG reactions to MetaCyc reactions. See the "Protein" tab of each gene for more information