MetaCyc Pathway: felinine and 3-methyl-3-sulfanylbutan-1-ol biosynthesis in Pseudomonas fluorescens FW300-N1B4

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Reactions and Genes Cobalt chloride 0.3125 mM
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3-(glutathion-S-yl)-(3-methyl)butanol + H2O→S-(3-methylbutan-3-yl)-L-cysteinylglycine + L-glutamate (EC 3.4.19.13)
No genes
S-(3-methylbutan-3-yl)-L-cysteinylglycine + H2O→felinine + glycine (EC 3.4.13.23)
No genes
Cysteine-S-conjugate beta-lyase:
felinine→2-aminoprop-2-enoate + 3-methyl-3-sulfanylbutan-1-ol (EC 4.4.1.13)
No genes
Tryptophanase:
2-aminoprop-2-enoate→2-iminopropanoate (spontaneous) (EC 4.1.99.1; 4.1.99.2; 4.3.1.13; 4.3.1.17; 4.3.1.18; 4.3.3.8; 4.4.1.1; 4.4.1.13; 4.4.1.15; 4.4.1.25; 4.4.1.28; 4.4.1.35; 4.5.1.2)
Pf1N1B4_1013 +2.2
Pf1N1B4_1692 +0.8
Pf1N1B4_2423 -0.0
Pf1N1B4_4257 -1.0
Pf1N1B4_4890 +0.6
Pf1N1B4_5639 -0.4
Pf1N1B4_622 -0.1
2-iminopropanoate + H2O→pyruvate + ammonium (spontaneous) (EC 3.5.99.10; 4.1.99.1; 4.1.99.2; 4.3.1.13; 4.3.1.17; 4.3.1.18; 4.3.3.8; 4.4.1.1; 4.4.1.13; 4.4.1.15; 4.4.1.25; 4.4.1.28; 4.4.1.35; 4.5.1.2)
Pf1N1B4_1013 +2.2
Pf1N1B4_1692 +0.8
Pf1N1B4_2112 -1.2
Pf1N1B4_2423 -0.0
Pf1N1B4_4257 -1.0
Pf1N1B4_4890 +0.6
Pf1N1B4_5639 -0.4
Pf1N1B4_622 -0.1

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Candidate genes for each reaction are identified from best hits to MetaCyc; by matching EC numbers (which are assigned by TIGRFam, SEED, or best hits to KEGG); or by matching SEED roles to KEGG reactions to MetaCyc reactions. See the "Protein" tab of each gene for more information