MetaCyc Pathway: reductive TCA cycle II in Burkholderia phytofirmans PsJN

Reactions and Genes	Paraquat dichloride 0.064 mg/ml
2-oxoglutarate synthase (in reverse): succinyl-CoA + CO2 + H+ + 2 a reduced ferredoxin [iron-sulfur] cluster→2-oxoglutarate + coenzyme A + 2 an oxidized ferredoxin [iron-sulfur] cluster (EC 1.2.7.3)
No genes
2-oxoglutarate carboxylase: 2-oxoglutarate + ATP + CO2 + H2O→oxalosuccinate + ADP + 2 H+ + phosphate (EC 6.4.1.7)
No genes
oxalosuccinate + NADH + H+→D-threo-isocitrate + NAD+
No genes
Aconitate hydratase (in reverse): D-threo-isocitrate→cis-aconitate + H2O (EC 4.2.1.3)
BPHYT_RS10160	-0.2
BPHYT_RS30145	-0.2
BPHYT_RS33980	-1.8
Aconitate hydratase (in reverse): cis-aconitate + H2O→citrate (EC 4.2.1.3)
BPHYT_RS10160	-0.2
BPHYT_RS30145	-0.2
BPHYT_RS33980	-1.8
Citrate--CoA ligase: citrate + ATP + coenzyme A→(3S)-citryl-CoA + ADP + phosphate (EC 6.2.1.18)
No genes
Citryl-CoA lyase: (3S)-citryl-CoA→acetyl-CoA + oxaloacetate (EC 4.1.3.34)
BPHYT_RS03015	-0.2
BPHYT_RS32670	-0.4
BPHYT_RS33960	-0.3
Malate dehydrogenase (in reverse): oxaloacetate + NADH + H+→(S)-malate + NAD+ (EC 1.1.1.37; 1.1.1.38)
BPHYT_RS27110	+0.1
BPHYT_RS28460	-0.3
BPHYT_RS33955	N.D.
Pyruvate synthase (in reverse): acetyl-CoA + CO2 + H+ + 2 a reduced ferredoxin [iron-sulfur] cluster→pyruvate + coenzyme A + 2 an oxidized ferredoxin [iron-sulfur] cluster (EC 1.2.7.1)
No genes
Fumarate hydratase: (S)-malate→fumarate + H2O (EC 4.2.1.2)
BPHYT_RS06985	+1.7
BPHYT_RS14615	-0.0
Succinate dehydrogenase (ubiquinone): fumarate[in] + a menaquinol→succinate[in] + a menaquinone (EC 1.3.5.1)
No genes
Succinate--CoA ligase (ADP-forming): succinate + ATP + coenzyme A→succinyl-CoA + ADP + phosphate (EC 6.2.1.5)
BPHYT_RS16340	-0.8
BPHYT_RS16345	-0.3

Candidate genes for each reaction are identified from best hits to MetaCyc; by matching EC numbers (which are assigned by TIGRFam, SEED, or best hits to KEGG); or by matching SEED roles to KEGG reactions to MetaCyc reactions. See the "Protein" tab of each gene for more information