MetaCyc Pathway: superpathway of L-isoleucine biosynthesis I in Sinorhizobium meliloti 1021

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Reactions and Genes NAG (C)
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Aspartate transaminase (in reverse):
oxaloacetate + L-glutamate→L-aspartate + 2-oxoglutarate (EC 2.6.1.1)
SM_b20039 -0.2
SMa0387 -0.1
SMa1495 -0.1
SMc00294 -4.7
SMc00490 -1.5
SMc01406 +0.2
SMc01578 -3.7
SMc01969 -0.1
SMc02251 -0.1
SMc02262 +0.0
SMc02393 +0.1
SMc04326 -0.1
SMc04386 -0.4
Aspartate kinase:
L-aspartate + ATP→L-aspartyl-4-phosphate + ADP (EC 2.7.2.4)
SMc02438 N.D.
Aspartate-semialdehyde dehydrogenase (in reverse):
L-aspartyl-4-phosphate + NADPH + H+→L-aspartate 4-semialdehyde + NADP+ + phosphate (EC 1.2.1.11)
SMc04410 N.D.
Homoserine dehydrogenase (in reverse):
L-aspartate 4-semialdehyde + NAD(P)H + H+→L-homoserine + NAD(P)+ (EC 1.1.1.3)
SMc00293 -6.5
Homoserine kinase:
L-homoserine + ATP→O-phospho-L-homoserine + ADP + H+ (EC 2.7.1.39)
SMa1760 -0.1
SMc00017 -4.4
Threonine synthase:
O-phospho-L-homoserine + H2O→L-threonine + phosphate (EC 4.2.3.1)
SMa0485 +0.0
SMc00077 N.D.
Threonine ammonia-lyase:
L-threonine→(Z)-2-aminobutenoate + H2O (EC 4.3.1.19)
SM_b20432 -0.0
SMa1872 -0.1
SMc00936 +0.2
1-aminocyclopropane-1-carboxylate deaminase:
(Z)-2-aminobutenoate→2-iminobutanoate (spontaneous) (EC 3.5.99.7; 4.3.1.19; 4.4.1.1; 4.4.1.11; 4.4.1.2)
SM_b20432 -0.0
SMa1872 -0.1
SMc00936 +0.2
SMc01666 +0.2
2-iminobutanoate + H2O→2-oxobutanoate + ammonium (spontaneous) (EC 3.5.99.10; 3.5.99.7; 4.3.1.19; 4.4.1.1; 4.4.1.11; 4.4.1.2)
SM_b20432 -0.0
SMa1872 -0.1
SMc00936 +0.2
SMc01666 +0.2
Acetolactate synthase:
2-oxobutanoate + H+ + pyruvate→(S)-2-aceto-2-hydroxybutanoate + CO2 (EC 2.2.1.6)
SM_b20400 +0.1
SMa0958 -0.0
SMa2211 -0.2
SMc01430 -4.0
SMc01431 -7.2
SMc02263 -0.1
SMc04455 +0.1
Ketol-acid reductoisomerase (in reverse):
(S)-2-aceto-2-hydroxybutanoate + NADPH + H+→(R)-2,3-dihydroxy-3-methylpentanoate + NADP+ (EC 1.1.1.86)
SMc04346 -3.3
Dihydroxy-acid dehydratase:
(R)-2,3-dihydroxy-3-methylpentanoate→(3S)-3-methyl-2-oxopentanoate + H2O (EC 4.2.1.9)
SM_b20115 -0.1
SM_b20890 -0.1
SMa0235 -0.2
SMc00884 -0.3
SMc04045 -7.6
SMc04144 +0.0
Branched-chain-amino-acid transaminase (in reverse):
(3S)-3-methyl-2-oxopentanoate + L-glutamate→L-isoleucine + 2-oxoglutarate (EC 2.6.1.42)
SMc00042 +0.0
SMc02896 +0.2

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Candidate genes for each reaction are identified from best hits to MetaCyc; by matching EC numbers (which are assigned by TIGRFam, SEED, or best hits to KEGG); or by matching SEED roles to KEGG reactions to MetaCyc reactions. See the "Protein" tab of each gene for more information