MetaCyc Pathway: 2-oxoisovalerate decarboxylation to isobutanoyl-CoA in Sinorhizobium meliloti 1021

Reactions and Genes	L-Leucine (C)
2-oxoisovalerate dehydrogenase (acylating): a [BCAA dehydrogenase E2 protein] N6-lipoyl-L-lysine + 3-methyl-2-oxobutanoate + H+→an [apo BCAA dehydrogenase E2 protein] N6-S-[2-methylpropanoyl]dihydrolipoyl-L-lysine + CO2 (EC 1.2.1.25; 1.2.4.4)
SM_b20020	+0.1
SMc03201	-4.0
SMc03202	-3.6
2-oxoisovalerate dehydrogenase (acylating) (in reverse): an [apo BCAA dehydrogenase E2 protein] N6-S-[2-methylpropanoyl]dihydrolipoyl-L-lysine + coenzyme A→an [apo BCAA dehydrogenase E2 protein] N6-dihydrolipoyl-L-lysine + isobutanoyl-CoA (EC 1.2.1.25; 2.3.1.168)
SM_b20019	+0.1
SMc03203	-3.8
2-oxoisovalerate dehydrogenase (acylating): an [apo BCAA dehydrogenase E2 protein] N6-dihydrolipoyl-L-lysine + NAD+→a [BCAA dehydrogenase E2 protein] N6-lipoyl-L-lysine + NADH + H+ (EC 1.2.1.25; 1.8.1.4)
SMc01035	N.D.
SMc02487	N.D.
SMc03204	-2.9

Candidate genes for each reaction are identified from best hits to MetaCyc; by matching EC numbers (which are assigned by TIGRFam, SEED, or best hits to KEGG); or by matching SEED roles to KEGG reactions to MetaCyc reactions. See the "Protein" tab of each gene for more information