MetaCyc Pathway: L-valine degradation I in Pseudomonas fluorescens FW300-N2E2

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Reactions and Genes L-Isoleucine (C)
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Branched-chain-amino-acid transaminase:
L-valine + 2-oxoglutarate→3-methyl-2-oxobutanoate + L-glutamate (EC 2.6.1.42)
Pf6N2E2_477 -6.2
2-oxoisovalerate dehydrogenase (acylating):
3-methyl-2-oxobutanoate + coenzyme A + NAD+→isobutanoyl-CoA + CO2 + NADH (EC 1.2.1.25)
No genes
isobutanoyl-CoA + an oxidized electron-transfer flavoprotein + H+→methylacrylyl-CoA + a reduced electron-transfer flavoprotein (EC 1.3.8.5)
Pf6N2E2_1146 -2.9
methylacrylyl-CoA + H2O→(S)-3-hydroxy-isobutanoyl-CoA (EC 4.2.1.150)
Pf6N2E2_1013 -0.1
Pf6N2E2_1046 -0.1
Pf6N2E2_1147 -6.3
Pf6N2E2_1269 -0.1
Pf6N2E2_1834 -0.1
Pf6N2E2_1835 -0.2
Pf6N2E2_1840 +0.0
Pf6N2E2_1922 -0.3
Pf6N2E2_1934 -0.1
Pf6N2E2_1935 -0.2
Pf6N2E2_2290 N.D.
Pf6N2E2_2773 +0.0
Pf6N2E2_3894 +0.0
Pf6N2E2_5493 -0.2
3-hydroxyisobutyryl-CoA hydrolase:
(S)-3-hydroxy-isobutanoyl-CoA + H2O→(S)-3-hydroxy-isobutanoate + coenzyme A + H+ (EC 3.1.2.4)
Pf6N2E2_1149 +0.1
3-hydroxyisobutyrate dehydrogenase:
(S)-3-hydroxy-isobutanoate + NAD+→(S)-methylmalonate-semialdehyde + NADH + H+ (EC 1.1.1.31)
Pf6N2E2_1833 +0.1
Pf6N2E2_1850 -0.0
Pf6N2E2_1922 -0.3
Pf6N2E2_2290 N.D.
Pf6N2E2_3451 -0.5
Pf6N2E2_3894 +0.0
Pf6N2E2_6000 +0.1
(S)-3-amino-2-methylpropionate transaminase (in reverse):
(S)-methylmalonate-semialdehyde + L-glutamate→(S)-3-amino-2-methylpropanoate + 2-oxoglutarate (EC 2.6.1.22)
No genes
Methylmalonate-semialdehyde dehydrogenase (acylating):
(S)-methylmalonate-semialdehyde + coenzyme A + NAD+ + H2O→propanoyl-CoA + hydrogen carbonate + NADH + H+ (EC 1.2.1.27)
Pf6N2E2_3452 -0.1
Pf6N2E2_3462 -0.2
Pf6N2E2_515 +0.1

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Candidate genes for each reaction are identified from best hits to MetaCyc; by matching EC numbers (which are assigned by TIGRFam, SEED, or best hits to KEGG); or by matching SEED roles to KEGG reactions to MetaCyc reactions. See the "Protein" tab of each gene for more information