MetaCyc Pathway: valproate β-oxidation in Pseudomonas fluorescens FW300-N2E2

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Reactions and Genes L-Isoleucine (C)
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Butyrate--CoA ligase:
valproate + ATP + coenzyme A→valproyl-CoA + AMP + diphosphate (EC 6.2.1.2)
No genes
valproyl-CoA + an oxidized electron-transfer flavoprotein + H+→(2E)-2-propylpent-2-enoyl-CoA + a reduced electron-transfer flavoprotein (EC 1.3.8.5)
Pf6N2E2_1146 -2.9
Enoyl-CoA hydratase (in reverse):
(2E)-2-propylpent-2-enoyl-CoA + H2O→(3S)-3-hydroxy-2-propylpentanoyl-CoA (EC 4.2.1.17)
Pf6N2E2_1013 -0.1
Pf6N2E2_1046 -0.1
Pf6N2E2_1147 -6.3
Pf6N2E2_1149 +0.1
Pf6N2E2_1269 -0.1
Pf6N2E2_1833 +0.1
Pf6N2E2_1834 -0.1
Pf6N2E2_1835 -0.2
Pf6N2E2_1840 +0.0
Pf6N2E2_1850 -0.0
Pf6N2E2_1922 -0.3
Pf6N2E2_1934 -0.1
Pf6N2E2_1935 -0.2
Pf6N2E2_2290 N.D.
Pf6N2E2_2773 +0.0
Pf6N2E2_3894 +0.0
Pf6N2E2_5493 -0.2
Pf6N2E2_6000 +0.1
Pf6N2E2_6004 N.D.
Pf6N2E2_742 -0.0
3-hydroxyacyl-CoA dehydrogenase:
(3S)-3-hydroxy-2-propylpentanoyl-CoA + NAD+→3-oxo-2-propylpentanoyl-CoA + NADH + H+ (EC 1.1.1.35)
Pf6N2E2_1833 +0.1
Pf6N2E2_1850 -0.0
Pf6N2E2_1922 -0.3
Pf6N2E2_2290 N.D.
Pf6N2E2_3894 +0.0
Pf6N2E2_4690 -0.2
Pf6N2E2_6000 +0.1
Acetyl-CoA C-acyltransferase (in reverse):
3-oxo-2-propylpentanoyl-CoA + coenzyme A→pentanoyl-CoA + propanoyl-CoA (EC 2.3.1.16)
Pf6N2E2_1145 N.D.
Pf6N2E2_1675 +0.0
Pf6N2E2_1836 +0.0
Pf6N2E2_1851 -0.1
Pf6N2E2_2113 -0.2
Pf6N2E2_2289 N.D.
Pf6N2E2_2539 -0.0
Pf6N2E2_2835 +0.4
Pf6N2E2_3492 -0.2
Pf6N2E2_5999 -0.1
pentanoyl-CoA + an oxidized electron-transfer flavoprotein + H+→(2E)-pent-2-enoyl-CoA + a reduced electron-transfer flavoprotein (EC 1.3.8.1)
Pf6N2E2_3686 -0.2
(2E)-pent-2-enoyl-CoA + H2O→(3S)-3-hydroxypentanoyl-CoA
No genes
3-hydroxyacyl-CoA dehydrogenase:
(3S)-3-hydroxypentanoyl-CoA + NAD+→3-oxopentanoyl-CoA + NADH + H+ (EC 1.1.1.35)
Pf6N2E2_1833 +0.1
Pf6N2E2_1850 -0.0
Pf6N2E2_1922 -0.3
Pf6N2E2_2290 N.D.
Pf6N2E2_3894 +0.0
Pf6N2E2_4690 -0.2
Pf6N2E2_6000 +0.1
Acetyl-CoA C-acyltransferase (in reverse):
3-oxopentanoyl-CoA + coenzyme A→propanoyl-CoA + acetyl-CoA (EC 2.3.1.16; 2.3.1.9)
Pf6N2E2_1145 N.D.
Pf6N2E2_1675 +0.0
Pf6N2E2_1836 +0.0
Pf6N2E2_1851 -0.1
Pf6N2E2_1923 +0.2
Pf6N2E2_2113 -0.2
Pf6N2E2_2289 N.D.
Pf6N2E2_2539 -0.0
Pf6N2E2_2835 +0.4
Pf6N2E2_3492 -0.2
Pf6N2E2_5999 -0.1

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Candidate genes for each reaction are identified from best hits to MetaCyc; by matching EC numbers (which are assigned by TIGRFam, SEED, or best hits to KEGG); or by matching SEED roles to KEGG reactions to MetaCyc reactions. See the "Protein" tab of each gene for more information