MetaCyc Pathway: pyruvate fermentation to butanol II (engineered) in Pseudomonas fluorescens FW300-N2E2

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Reactions and Genes L-Isoleucine (C)
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Acetyl-CoA C-acyltransferase:
2 acetyl-CoA→acetoacetyl-CoA + coenzyme A (EC 2.3.1.16; 2.3.1.9)
Pf6N2E2_1145 N.D.
Pf6N2E2_1675 +0.0
Pf6N2E2_1836 +0.0
Pf6N2E2_1851 -0.1
Pf6N2E2_1923 +0.2
Pf6N2E2_2113 -0.2
Pf6N2E2_2289 N.D.
Pf6N2E2_2539 -0.0
Pf6N2E2_2835 +0.4
Pf6N2E2_3492 -0.2
Pf6N2E2_5999 -0.1
3-hydroxyacyl-CoA dehydrogenase (in reverse):
acetoacetyl-CoA + NADH + H+→(S)-3-hydroxybutanoyl-CoA + NAD+ (EC 1.1.1.35)
Pf6N2E2_1722 -0.2
Pf6N2E2_1833 +0.1
Pf6N2E2_1850 -0.0
Pf6N2E2_1922 -0.3
Pf6N2E2_2290 N.D.
Pf6N2E2_3894 +0.0
Pf6N2E2_4690 -0.2
Pf6N2E2_6000 +0.1
(S)-3-hydroxybutanoyl-CoA→crotonyl-CoA + H2O (EC 4.2.1.150)
Pf6N2E2_1013 -0.1
Pf6N2E2_1046 -0.1
Pf6N2E2_1834 -0.1
Pf6N2E2_1835 -0.2
Pf6N2E2_1840 +0.0
Pf6N2E2_1922 -0.3
Pf6N2E2_1934 -0.1
Pf6N2E2_1935 -0.2
Pf6N2E2_2290 N.D.
Pf6N2E2_2773 +0.0
Pf6N2E2_3894 +0.0
Pf6N2E2_5493 -0.2
Trans-2-enoyl-CoA reductase (NAD(+)) (in reverse):
crotonyl-CoA + NADH + H+→butanoyl-CoA + NAD+ (EC 1.3.1.44)
Pf6N2E2_1022 +0.4
Pf6N2E2_1146 -2.9
Pf6N2E2_1148 +0.2
Pf6N2E2_1855 -0.1
Pf6N2E2_5333 +0.2
Pf6N2E2_5924 +0.1
Butanal dehydrogenase (in reverse):
butanoyl-CoA + NAD(P)H + H+→1-butanal + coenzyme A + NAD(P)+ (EC 1.2.1.57)
No genes
1-butanal + NADH + H+→butan-1-ol + NAD+
No genes

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Candidate genes for each reaction are identified from best hits to MetaCyc; by matching EC numbers (which are assigned by TIGRFam, SEED, or best hits to KEGG); or by matching SEED roles to KEGG reactions to MetaCyc reactions. See the "Protein" tab of each gene for more information