MetaCyc Pathway: (R)- and (S)-3-hydroxybutanoate biosynthesis (engineered) in Burkholderia phytofirmans PsJN

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Reactions and Genes L-Tryptophan (C)
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Acetyl-CoA C-acyltransferase:
2 acetyl-CoA→acetoacetyl-CoA + coenzyme A (EC 2.3.1.16; 2.3.1.9)
BPHYT_RS02230 +0.1
BPHYT_RS03230 +0.3
BPHYT_RS08275 +0.0
BPHYT_RS09150 -1.3
BPHYT_RS09180 -0.0
BPHYT_RS09565 +0.6
BPHYT_RS17345 -0.2
BPHYT_RS21955 -0.6
BPHYT_RS29385 -0.8
3-hydroxyacyl-CoA dehydrogenase (in reverse):
acetoacetyl-CoA + NADH + H+→(S)-3-hydroxybutanoyl-CoA + NAD+ (EC 1.1.1.35)
BPHYT_RS03225 +0.2
BPHYT_RS06760 +0.3
BPHYT_RS09560 +0.0
BPHYT_RS13545 -0.4
BPHYT_RS28020 N.D.
BPHYT_RS34155 +0.5
Acetoacetyl-CoA reductase (in reverse):
acetoacetyl-CoA + NADPH + H+→(3R)-3-hydroxybutanoyl-CoA + NADP+ (EC 1.1.1.36)
BPHYT_RS00860 +0.1
BPHYT_RS09155 -0.3
BPHYT_RS15135 +0.7
BPHYT_RS27640 -0.2
BPHYT_RS29090 -0.1
(S)-3-hydroxybutanoyl-CoA + H2O→(S)-3-hydroxybutanoate + coenzyme A + H+
No genes
(3R)-3-hydroxybutanoyl-CoA + H2O→(R)-3-hydroxybutanoate + coenzyme A + H+
No genes

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Candidate genes for each reaction are identified from best hits to MetaCyc; by matching EC numbers (which are assigned by TIGRFam, SEED, or best hits to KEGG); or by matching SEED roles to KEGG reactions to MetaCyc reactions. See the "Protein" tab of each gene for more information