MetaCyc Pathway: L-isoleucine degradation I in Burkholderia phytofirmans PsJN

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Reactions and Genes L-Tryptophan (C)
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Branched-chain-amino-acid transaminase:
L-isoleucine + 2-oxoglutarate→(3S)-3-methyl-2-oxopentanoate + L-glutamate (EC 2.6.1.42)
BPHYT_RS16285 -0.0
2-oxoisovalerate dehydrogenase (acylating):
(3S)-3-methyl-2-oxopentanoate + coenzyme A + NAD+→(2S)-2-methylbutanoyl-CoA + CO2 + NADH (EC 1.2.1.25)
No genes
(2S)-2-methylbutanoyl-CoA + an oxidized electron-transfer flavoprotein + H+→(E)-2-methylcrotonoyl-CoA + a reduced electron-transfer flavoprotein (EC 1.3.8.5)
No genes
(E)-2-methylcrotonoyl-CoA + H2O→(2S,3S)-3-hydroxy-2-methylbutanoyl-CoA (EC 4.2.1.150)
BPHYT_RS02345 -0.2
BPHYT_RS03225 +0.2
BPHYT_RS03235 +0.2
BPHYT_RS03825 N.D.
BPHYT_RS08375 N.D.
BPHYT_RS08630 +0.2
BPHYT_RS09560 +0.0
BPHYT_RS10815 N.D.
BPHYT_RS11780 N.D.
BPHYT_RS13545 -0.4
BPHYT_RS14830 +0.1
BPHYT_RS25360 N.D.
BPHYT_RS25695 N.D.
BPHYT_RS27105 +0.2
BPHYT_RS27895 -0.3
BPHYT_RS28020 N.D.
BPHYT_RS28620 +0.5
BPHYT_RS35465 -2.4
3-hydroxy-2-methylbutyryl-CoA dehydrogenase:
(2S,3S)-3-hydroxy-2-methylbutanoyl-CoA + NAD+→2-methylacetoacetyl-CoA + NADH + H+ (EC 1.1.1.178)
BPHYT_RS03225 +0.2
BPHYT_RS06760 +0.3
BPHYT_RS09560 +0.0
BPHYT_RS13545 -0.4
BPHYT_RS15890 -1.5
BPHYT_RS28020 N.D.
BPHYT_RS34155 +0.5
Acetyl-CoA C-acyltransferase (in reverse):
2-methylacetoacetyl-CoA + coenzyme A→acetyl-CoA + propanoyl-CoA (EC 2.3.1.16)
BPHYT_RS02230 +0.1
BPHYT_RS03230 +0.3
BPHYT_RS08275 +0.0
BPHYT_RS09150 -1.3
BPHYT_RS09180 -0.0
BPHYT_RS09565 +0.6
BPHYT_RS17345 -0.2
BPHYT_RS21955 -0.6
BPHYT_RS29385 -0.8

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Candidate genes for each reaction are identified from best hits to MetaCyc; by matching EC numbers (which are assigned by TIGRFam, SEED, or best hits to KEGG); or by matching SEED roles to KEGG reactions to MetaCyc reactions. See the "Protein" tab of each gene for more information