MetaCyc Pathway: superpathway of L-isoleucine biosynthesis I in Pseudomonas fluorescens FW300-N2C3

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Reactions and Genes L-Arabinose (C)
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Aspartate transaminase (in reverse):
oxaloacetate + L-glutamate→L-aspartate + 2-oxoglutarate (EC 2.6.1.1)
AO356_01275 +0.3
AO356_03090 -0.0
AO356_03985 -0.1
AO356_06565 -0.1
AO356_12070 -0.0
AO356_12595 -0.0
AO356_16875 -0.1
AO356_19495 -0.0
AO356_19865 -0.0
AO356_19905 +0.0
AO356_20345 -0.1
AO356_22880 -0.1
AO356_24005 +0.2
AO356_24115 -0.1
AO356_24720 +0.0
AO356_24975 -0.1
AO356_25355 +0.1
Aspartate kinase:
L-aspartate + ATP→L-aspartyl-4-phosphate + ADP (EC 2.7.2.4)
AO356_18775 -0.3
Aspartate-semialdehyde dehydrogenase (in reverse):
L-aspartyl-4-phosphate + NADPH + H+→L-aspartate 4-semialdehyde + NADP+ + phosphate (EC 1.2.1.11)
AO356_04970 -0.2
AO356_21420 N.D.
AO356_21425 -0.3
Homoserine dehydrogenase (in reverse):
L-aspartate 4-semialdehyde + NAD(P)H + H+→L-homoserine + NAD(P)+ (EC 1.1.1.3)
AO356_06375 N.D.
AO356_15140 +0.5
AO356_27140 -0.1
Homoserine kinase:
L-homoserine + ATP→O-phospho-L-homoserine + ADP + H+ (EC 2.7.1.39)
AO356_11475 N.D.
AO356_20835 +0.1
Threonine synthase:
O-phospho-L-homoserine + H2O→L-threonine + phosphate (EC 4.2.3.1)
AO356_06370 N.D.
AO356_15130 +0.2
AO356_16855 -0.2
AO356_27150 +0.3
Threonine ammonia-lyase:
L-threonine→(Z)-2-aminobutenoate + H2O (EC 4.3.1.19)
AO356_10330 -0.9
AO356_23665 -0.0
AO356_27710 -0.2
1-aminocyclopropane-1-carboxylate deaminase:
(Z)-2-aminobutenoate→2-iminobutanoate (spontaneous) (EC 3.5.99.7; 4.3.1.19; 4.4.1.1; 4.4.1.11; 4.4.1.2)
AO356_01695 +0.4
AO356_10330 -0.9
AO356_19910 -0.0
AO356_23665 -0.0
AO356_27130 +0.2
AO356_27710 -0.2
AO356_29420 -0.2
2-iminobutanoate + H2O→2-oxobutanoate + ammonium (spontaneous) (EC 3.5.99.10; 3.5.99.7; 4.3.1.19; 4.4.1.1; 4.4.1.11; 4.4.1.2)
AO356_01695 +0.4
AO356_10330 -0.9
AO356_12475 -0.2
AO356_19910 -0.0
AO356_23665 -0.0
AO356_27130 +0.2
AO356_27710 -0.2
AO356_29420 -0.2
Acetolactate synthase:
2-oxobutanoate + H+ + pyruvate→(S)-2-aceto-2-hydroxybutanoate + CO2 (EC 2.2.1.6)
AO356_16170 -3.4
AO356_16175 -3.7
AO356_17755 -0.0
AO356_28690 +0.3
Ketol-acid reductoisomerase (in reverse):
(S)-2-aceto-2-hydroxybutanoate + NADPH + H+→(R)-2,3-dihydroxy-3-methylpentanoate + NADP+ (EC 1.1.1.86)
AO356_16180 -3.6
Dihydroxy-acid dehydratase:
(R)-2,3-dihydroxy-3-methylpentanoate→(3S)-3-methyl-2-oxopentanoate + H2O (EC 4.2.1.9)
AO356_13250 -3.7
AO356_24585 -5.4
AO356_28760 -0.4
Branched-chain-amino-acid transaminase (in reverse):
(3S)-3-methyl-2-oxopentanoate + L-glutamate→L-isoleucine + 2-oxoglutarate (EC 2.6.1.42)
AO356_22970 -0.5

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Candidate genes for each reaction are identified from best hits to MetaCyc; by matching EC numbers (which are assigned by TIGRFam, SEED, or best hits to KEGG); or by matching SEED roles to KEGG reactions to MetaCyc reactions. See the "Protein" tab of each gene for more information