MetaCyc Pathway: toluene degradation III (aerobic) (via p-cresol) in Burkholderia phytofirmans PsJN

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Reactions and Genes L-Phenylalanine (C)
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toluene + NADH + dioxygen + H+→4-methylphenol + NAD+ + H2O (EC 1.14.13.236)
No genes
4-methylphenol + 2 an oxidized azurin + H2O→4-hydroxybenzyl alcohol + 2 H+ + 2 a reduced azurin (EC 1.17.9.1)
No genes
4-hydroxybenzyl alcohol + 2 an oxidized azurin→4-hydroxybenzaldehyde + 2 H+ + 2 a reduced azurin (EC 1.17.9.1)
No genes
4-hydroxybenzaldehyde + NADP+ + H2O→4-hydroxybenzoate + NADPH + 2 H+ (EC 1.2.1.96)
No genes
4-hydroxybenzoate 3-monooxygenase:
4-hydroxybenzoate + NADPH + dioxygen + H+→protocatechuate + NADP+ + H2O (EC 1.14.13.2)
BPHYT_RS11060 +0.4
Protocatechuate 3,4-dioxygenase:
protocatechuate + dioxygen→3-carboxy-cis,cis-muconate + 2 H+ (EC 1.13.11.3)
BPHYT_RS33455 +0.1
BPHYT_RS33460 -0.1
3-carboxy-cis,cis-muconate cycloisomerase (in reverse):
3-carboxy-cis,cis-muconate + H+→(2-carboxy-2,5-dihydro-5-oxofuran-2-yl)-acetate (EC 5.5.1.2)
BPHYT_RS21425 -0.1
4-carboxymuconolactone decarboxylase:
(2-carboxy-2,5-dihydro-5-oxofuran-2-yl)-acetate + H+→(4,5-dihydro-5-oxofuran-2-yl)-acetate + CO2 (EC 4.1.1.44)
BPHYT_RS09010 +0.1
BPHYT_RS21435 N.D.
BPHYT_RS27085 -0.0
BPHYT_RS27165 -0.1
BPHYT_RS28360 +0.6
BPHYT_RS28365 -0.0
3-oxoadipate enol-lactonase:
(4,5-dihydro-5-oxofuran-2-yl)-acetate + H2O→3-oxoadipate + H+ (EC 3.1.1.24)
BPHYT_RS02320 +0.0
BPHYT_RS21430 N.D.
BPHYT_RS34955 N.D.
3-oxoadipate CoA-transferase:
3-oxoadipate + succinyl-CoA→3-oxoadipoyl-CoA + succinate (EC 2.8.3.6)
BPHYT_RS21415 -0.4
BPHYT_RS21420 -0.1
3-oxoadipyl-CoA thiolase (in reverse):
3-oxoadipoyl-CoA + coenzyme A→acetyl-CoA + succinyl-CoA (EC 2.3.1.174)
BPHYT_RS17345 -1.9
BPHYT_RS29385 -0.2

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Candidate genes for each reaction are identified from best hits to MetaCyc; by matching EC numbers (which are assigned by TIGRFam, SEED, or best hits to KEGG); or by matching SEED roles to KEGG reactions to MetaCyc reactions. See the "Protein" tab of each gene for more information