MetaCyc Pathway: glycerol degradation to butanol in Sinorhizobium meliloti 1021

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Reactions and Genes Glycerol (C)
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Glycerol dehydrogenase:
glycerol + NAD+→dihydroxyacetone + NADH + H+ (EC 1.1.1.6)
SMc02038 -0.1
Glycerone kinase:
dihydroxyacetone + ATP→glycerone phosphate + ADP + H+ (EC 2.7.1.29)
SM_b20307 -0.4
SM_b20312 -0.0
SM_b20313 -0.1
SM_b20314 -0.3
SM_b20767 -0.1
Triose-phosphate isomerase (in reverse):
glycerone phosphate→D-glyceraldehyde 3-phosphate (EC 5.3.1.1)
SMc01023 -3.2
SMc01614 -0.3
Glyceraldehyde-3-phosphate dehydrogenase (phosphorylating):
D-glyceraldehyde 3-phosphate + NAD+ + phosphate→3-phospho-D-glyceroyl phosphate + NADH + H+ (EC 1.2.1.12)
SMc03979 -5.6
Phosphoglycerate kinase (in reverse):
3-phospho-D-glyceroyl phosphate + ADP→3-phospho-D-glycerate + ATP (EC 2.7.2.3)
SMc03981 -3.6
3-phospho-D-glycerate→2-phospho-D-glycerate (EC 5.4.2.12)
SMc02838 -1.8
Phosphopyruvate hydratase:
2-phospho-D-glycerate→phosphoenolpyruvate + H2O (EC 4.2.1.11)
SMc01028 -2.4
Pyruvate kinase (in reverse):
phosphoenolpyruvate + ADP + H+→pyruvate + ATP (EC 2.7.1.40)
SMc04005 -4.1
Pyruvate synthase:
pyruvate + coenzyme A + 2 an oxidized ferredoxin [iron-sulfur] cluster→acetyl-CoA + CO2 + H+ + 2 a reduced ferredoxin [iron-sulfur] cluster (EC 1.2.7.1)
No genes
Acetyl-CoA C-acyltransferase:
2 acetyl-CoA→acetoacetyl-CoA + coenzyme A (EC 2.3.1.16; 2.3.1.9)
SM_b20589 -0.2
SMa1450 +0.1
SMc00262 +0.0
SMc00966 -0.1
SMc02228 -2.4
SMc03879 +0.6
3-hydroxyacyl-CoA dehydrogenase (in reverse):
acetoacetyl-CoA + NADH + H+→(S)-3-hydroxybutanoyl-CoA + NAD+ (EC 1.1.1.35)
SM_b21632 -0.1
SMc00727 -0.2
SMc01638 +0.2
SMc02227 -2.1
(S)-3-hydroxybutanoyl-CoA→crotonyl-CoA + H2O (EC 4.2.1.150)
SM_b21632 -0.1
SM_b21633 +0.3
SMc00976 +0.4
SMc01638 +0.2
SMc01669 +0.2
SMc01806 -0.1
SMc04398 N.D.
crotonyl-CoA[cytosol] + 2 NADH[cytosol] + 2 an oxidized ferredoxin [iron-sulfur] cluster[cytosol]→butanoyl-CoA[cytosol] + 2 NAD+[cytosol] + 2 a reduced ferredoxin [iron-sulfur] cluster[cytosol] (EC 1.3.1.109)
No genes
Butanal dehydrogenase (in reverse):
butanoyl-CoA + NAD(P)H + H+→1-butanal + coenzyme A + NAD(P)+ (EC 1.2.1.57)
No genes
Alcohol dehydrogenase (NADP(+)) (in reverse):
1-butanal + NADPH + H+→butan-1-ol + NADP+ (EC 1.1.1.2)
SM_b20493 +0.0
1-butanal + NADH + H+→butan-1-ol + NAD+
No genes

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Candidate genes for each reaction are identified from best hits to MetaCyc; by matching EC numbers (which are assigned by TIGRFam, SEED, or best hits to KEGG); or by matching SEED roles to KEGG reactions to MetaCyc reactions. See the "Protein" tab of each gene for more information