MetaCyc Pathway: L-isoleucine degradation II in Pseudomonas fluorescens FW300-N2E2

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Reactions and Genes propionate (C)
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Branched-chain-amino-acid transaminase:
L-isoleucine + 2-oxoglutarate→(3S)-3-methyl-2-oxopentanoate + L-glutamate (EC 2.6.1.42)
Pf6N2E2_477 +0.1
Branched-chain-2-oxoacid decarboxylase:
(3S)-3-methyl-2-oxopentanoate + H+→2-methylbutanal + CO2 (EC 4.1.1.72)
No genes
Alcohol dehydrogenase (in reverse):
2-methylbutanal + NADH + H+→2-methylbutanol + NAD+ (EC 1.1.1.1)
Pf6N2E2_1380 N.D.
Pf6N2E2_1522 +0.3
Pf6N2E2_194 -0.2
Pf6N2E2_2802 -0.0
Pf6N2E2_3044 +0.2
Pf6N2E2_4819 -0.1
Pf6N2E2_5980 +0.1
Pf6N2E2_840 +1.1
Pf6N2E2_999 -0.2

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Candidate genes for each reaction are identified from best hits to MetaCyc; by matching EC numbers (which are assigned by TIGRFam, SEED, or best hits to KEGG); or by matching SEED roles to KEGG reactions to MetaCyc reactions. See the "Protein" tab of each gene for more information