MetaCyc Pathway: pyruvate fermentation to isobutanol (engineered) in Pseudomonas fluorescens FW300-N2C3

Add experiment(s):


Reactions and Genes D-Xylose (C)
remove
Acetolactate synthase:
2 pyruvate + H+→(S)-2-acetolactate + CO2 (EC 2.2.1.6)
AO356_16170 -3.9
AO356_16175 -4.0
AO356_17755 +0.0
AO356_28690 -0.4
Ketol-acid reductoisomerase (in reverse):
(S)-2-acetolactate + NADPH + H+→(2R)-2,3-dihydroxy-3-methylbutanoate + NADP+ (EC 1.1.1.86)
AO356_16180 -3.4
Dihydroxy-acid dehydratase:
(2R)-2,3-dihydroxy-3-methylbutanoate→3-methyl-2-oxobutanoate + H2O (EC 4.2.1.9)
AO356_13250 -3.6
AO356_24585 +0.2
AO356_28760 -1.5
Branched-chain-2-oxoacid decarboxylase:
3-methyl-2-oxobutanoate + H+→isobutanal + CO2 (EC 4.1.1.72)
No genes
Alcohol dehydrogenase (in reverse):
isobutanal + NADH + H+→isobutanol + NAD+ (EC 1.1.1.1)
AO356_00820 +0.1
AO356_04765 -0.0
AO356_05920 -0.0
AO356_14405 -0.2
AO356_20305 +0.3
AO356_28020 +0.2
AO356_29985 -0.2

Links:

Candidate genes for each reaction are identified from best hits to MetaCyc; by matching EC numbers (which are assigned by TIGRFam, SEED, or best hits to KEGG); or by matching SEED roles to KEGG reactions to MetaCyc reactions. See the "Protein" tab of each gene for more information