MetaCyc Pathway: anteiso-branched-chain fatty acid biosynthesis in Burkholderia phytofirmans PsJN

Add experiment(s):


Reactions and Genes D-Galactose (C)
remove
Branched-chain-amino-acid transaminase:
L-isoleucine + 2-oxoglutarate→(3S)-3-methyl-2-oxopentanoate + L-glutamate
(EC 2.6.1.42)
BPHYT_RS16285 +0.2
2-oxoisovalerate dehydrogenase (acylating):
(3S)-3-methyl-2-oxopentanoate + coenzyme A + NAD+→(2S)-2-methylbutanoyl-CoA + CO2 + NADH
(EC 1.2.1.25)
No genes
(2S)-2-methylbutanoyl-CoA + a malonyl-[acp] + H+→a (4S)-4-methyl-3-oxohhexanoyl-[acp] + CO2 + coenzyme A (EC 2.3.1.300)
No genes
3-oxoacyl-[acyl-carrier-protein] reductase (in reverse):
a (4S)-4-methyl-3-oxohhexanoyl-[acp] + NADPH + H+→a (4S,3R)-3-hydroxy-4-methylhexanoyl-[acp] + NADP+
(EC 1.1.1.100)
BPHYT_RS01050 -0.1
BPHYT_RS04770 +0.0
BPHYT_RS05365 -0.2
BPHYT_RS05385 +0.3
BPHYT_RS06420 N.D.
BPHYT_RS07065 -0.0
BPHYT_RS08285 N.D.
BPHYT_RS08365 -0.0
BPHYT_RS08890 -0.2
BPHYT_RS10170 +0.1
BPHYT_RS10200 N.D.
BPHYT_RS10595 -0.1
BPHYT_RS10630 +0.1
BPHYT_RS10735 N.D.
BPHYT_RS11845 N.D.
BPHYT_RS13055 -0.5
BPHYT_RS14425 N.D.
BPHYT_RS15135 +0.1
BPHYT_RS15890 -1.4
BPHYT_RS16040 N.D.
BPHYT_RS16940 -2.2
BPHYT_RS20510 N.D.
BPHYT_RS20625 +0.1
BPHYT_RS20775 +0.5
BPHYT_RS21600 +0.1
BPHYT_RS22135 -0.2
BPHYT_RS25030 -0.1
BPHYT_RS25415 N.D.
BPHYT_RS25655 +0.1
BPHYT_RS26470 +0.6
BPHYT_RS28250 N.D.
BPHYT_RS30440 +0.0
BPHYT_RS30465 -1.2
BPHYT_RS30795 -0.2
BPHYT_RS31385 +0.3
BPHYT_RS31405 N.D.
BPHYT_RS31610 +0.0
BPHYT_RS31900 -0.2
BPHYT_RS33140 N.D.
BPHYT_RS33705 -0.1
BPHYT_RS34945 N.D.
BPHYT_RS34965 N.D.
BPHYT_RS35505 -0.2
BPHYT_RS35675 -0.2
3-hydroxyoctanoyl-[acyl-carrier-protein] dehydratase:
a (4S,3R)-3-hydroxy-4-methylhexanoyl-[acp]→a (2E,4S)-4-methylhex-2-enoyl-[acp] + H2O
(EC 4.2.1.59)
BPHYT_RS12085 N.D.
Enoyl-[acyl-carrier-protein] reductase (NADH) (in reverse):
a (2E,4S)-4-methylhex-2-enoyl-[acp] + NADH + H+→a (4S)-4-methylhexanoyl-[acp] + NAD+
(EC 1.3.1.9)
BPHYT_RS01880 +0.0
BPHYT_RS12390 +0.2
BPHYT_RS20615 -0.0
BPHYT_RS30015 N.D.
BPHYT_RS33380 -0.2
Enoyl-[acyl-carrier-protein] reductase (NADPH, B-specific) (in reverse):
a (2E,4S)-4-methylhex-2-enoyl-[acp] + NADPH + H+→a (4S)-4-methylhexanoyl-[acp] + NADP+
(EC 1.3.1.10; 1.3.1.104; 1.3.1.39)
BPHYT_RS32935 -0.0
Beta-ketoacyl-acyl-carrier-protein synthase II:
a (4S)-4-methylhexanoyl-[acp] + a malonyl-[acp] + H+→a (6S)-6-methyl-3-oxooctanoyl-[acp] + a soluble [acyl-carrier protein] + CO2
(EC 2.3.1.179)
BPHYT_RS14415 N.D.
BPHYT_RS15130 +0.0
3-oxoacyl-[acyl-carrier-protein] reductase (in reverse):
a (6S)-6-methyl-3-oxooctanoyl-[acp] + NADPH + H+→a (3R,6S)-3-hydroxy-6-methyloctanoyl-[acp] + NADP+
(EC 1.1.1.100)
BPHYT_RS01050 -0.1
BPHYT_RS04770 +0.0
BPHYT_RS05365 -0.2
BPHYT_RS05385 +0.3
BPHYT_RS06420 N.D.
BPHYT_RS07065 -0.0
BPHYT_RS08285 N.D.
BPHYT_RS08365 -0.0
BPHYT_RS08890 -0.2
BPHYT_RS10170 +0.1
BPHYT_RS10200 N.D.
BPHYT_RS10595 -0.1
BPHYT_RS10630 +0.1
BPHYT_RS10735 N.D.
BPHYT_RS11845 N.D.
BPHYT_RS13055 -0.5
BPHYT_RS14425 N.D.
BPHYT_RS15135 +0.1
BPHYT_RS15890 -1.4
BPHYT_RS16040 N.D.
BPHYT_RS16940 -2.2
BPHYT_RS20510 N.D.
BPHYT_RS20625 +0.1
BPHYT_RS20775 +0.5
BPHYT_RS21600 +0.1
BPHYT_RS22135 -0.2
BPHYT_RS25030 -0.1
BPHYT_RS25415 N.D.
BPHYT_RS25655 +0.1
BPHYT_RS26470 +0.6
BPHYT_RS28250 N.D.
BPHYT_RS30440 +0.0
BPHYT_RS30465 -1.2
BPHYT_RS30795 -0.2
BPHYT_RS31385 +0.3
BPHYT_RS31405 N.D.
BPHYT_RS31610 +0.0
BPHYT_RS31900 -0.2
BPHYT_RS33140 N.D.
BPHYT_RS33705 -0.1
BPHYT_RS34945 N.D.
BPHYT_RS34965 N.D.
BPHYT_RS35505 -0.2
BPHYT_RS35675 -0.2
3-hydroxyoctanoyl-[acyl-carrier-protein] dehydratase:
a (3R,6S)-3-hydroxy-6-methyloctanoyl-[acp]→a (2E,6S)-6-methyloct-2-enoyl-[acp] + H2O
(EC 4.2.1.59)
BPHYT_RS12085 N.D.
Enoyl-[acyl-carrier-protein] reductase (NADH) (in reverse):
a (2E,6S)-6-methyloct-2-enoyl-[acp] + NADH + H+→a (6S)-6-methyloctanoyl-[acp] + NAD+
(EC 1.3.1.9)
BPHYT_RS01880 +0.0
BPHYT_RS12390 +0.2
BPHYT_RS20615 -0.0
BPHYT_RS30015 N.D.
BPHYT_RS33380 -0.2
Enoyl-[acyl-carrier-protein] reductase (NADPH, B-specific) (in reverse):
a (2E,6S)-6-methyloct-2-enoyl-[acp] + NADPH + H+→a (6S)-6-methyloctanoyl-[acp] + NADP+
(EC 1.3.1.10; 1.3.1.104)
BPHYT_RS32935 -0.0
Beta-ketoacyl-acyl-carrier-protein synthase II:
a (6S)-6-methyloctanoyl-[acp] + a malonyl-[acp] + H+→an (8S)-8-methyl-3-oxodecanoyl-[acp] + a soluble [acyl-carrier protein] + CO2
(EC 2.3.1.179)
BPHYT_RS14415 N.D.
BPHYT_RS15130 +0.0
3-oxoacyl-[acyl-carrier-protein] reductase (in reverse):
an (8S)-8-methyl-3-oxodecanoyl-[acp] + NADPH + H+→a (3R,8S)-3-hydroxy-8-methyldecanoyl-[acp] + NADP+
(EC 1.1.1.100)
BPHYT_RS01050 -0.1
BPHYT_RS04770 +0.0
BPHYT_RS05365 -0.2
BPHYT_RS05385 +0.3
BPHYT_RS06420 N.D.
BPHYT_RS07065 -0.0
BPHYT_RS08285 N.D.
BPHYT_RS08365 -0.0
BPHYT_RS08890 -0.2
BPHYT_RS10170 +0.1
BPHYT_RS10200 N.D.
BPHYT_RS10595 -0.1
BPHYT_RS10630 +0.1
BPHYT_RS10735 N.D.
BPHYT_RS11845 N.D.
BPHYT_RS13055 -0.5
BPHYT_RS14425 N.D.
BPHYT_RS15135 +0.1
BPHYT_RS15890 -1.4
BPHYT_RS16040 N.D.
BPHYT_RS16940 -2.2
BPHYT_RS20510 N.D.
BPHYT_RS20625 +0.1
BPHYT_RS20775 +0.5
BPHYT_RS21600 +0.1
BPHYT_RS22135 -0.2
BPHYT_RS25030 -0.1
BPHYT_RS25415 N.D.
BPHYT_RS25655 +0.1
BPHYT_RS26470 +0.6
BPHYT_RS28250 N.D.
BPHYT_RS30440 +0.0
BPHYT_RS30465 -1.2
BPHYT_RS30795 -0.2
BPHYT_RS31385 +0.3
BPHYT_RS31405 N.D.
BPHYT_RS31610 +0.0
BPHYT_RS31900 -0.2
BPHYT_RS33140 N.D.
BPHYT_RS33705 -0.1
BPHYT_RS34945 N.D.
BPHYT_RS34965 N.D.
BPHYT_RS35505 -0.2
BPHYT_RS35675 -0.2
3-hydroxyoctanoyl-[acyl-carrier-protein] dehydratase:
a (3R,8S)-3-hydroxy-8-methyldecanoyl-[acp]→a (2E,8S)-8-methyldec-2-enoyl-[acp] + H2O
(EC 4.2.1.59)
BPHYT_RS12085 N.D.
Enoyl-[acyl-carrier-protein] reductase (NADH) (in reverse):
a (2E,8S)-8-methyldec-2-enoyl-[acp] + NADH + H+→an (8S)-8-methyldecanoyl-[acyl-carrier protein] + NAD+
(EC 1.3.1.9)
BPHYT_RS01880 +0.0
BPHYT_RS12390 +0.2
BPHYT_RS20615 -0.0
BPHYT_RS30015 N.D.
BPHYT_RS33380 -0.2
Enoyl-[acyl-carrier-protein] reductase (NADPH, B-specific) (in reverse):
a (2E,8S)-8-methyldec-2-enoyl-[acp] + NADPH + H+→an (8S)-8-methyldecanoyl-[acyl-carrier protein] + NADP+
(EC 1.3.1.10; 1.3.1.104)
BPHYT_RS32935 -0.0
Beta-ketoacyl-acyl-carrier-protein synthase II:
an (8S)-8-methyldecanoyl-[acyl-carrier protein] + a malonyl-[acp] + H+→a (10S)-10-methyl-3-oxododecanoyl-[acp] + a soluble [acyl-carrier protein] + CO2
(EC 2.3.1.179)
BPHYT_RS14415 N.D.
BPHYT_RS15130 +0.0
3-oxoacyl-[acyl-carrier-protein] reductase (in reverse):
a (10S)-10-methyl-3-oxododecanoyl-[acp] + NADPH + H+→a (3R,10S)-3-hydroxy-10-methyldodecanoyl-[acp] + NADP+
(EC 1.1.1.100)
BPHYT_RS01050 -0.1
BPHYT_RS04770 +0.0
BPHYT_RS05365 -0.2
BPHYT_RS05385 +0.3
BPHYT_RS06420 N.D.
BPHYT_RS07065 -0.0
BPHYT_RS08285 N.D.
BPHYT_RS08365 -0.0
BPHYT_RS08890 -0.2
BPHYT_RS10170 +0.1
BPHYT_RS10200 N.D.
BPHYT_RS10595 -0.1
BPHYT_RS10630 +0.1
BPHYT_RS10735 N.D.
BPHYT_RS11845 N.D.
BPHYT_RS13055 -0.5
BPHYT_RS14425 N.D.
BPHYT_RS15135 +0.1
BPHYT_RS15890 -1.4
BPHYT_RS16040 N.D.
BPHYT_RS16940 -2.2
BPHYT_RS20510 N.D.
BPHYT_RS20625 +0.1
BPHYT_RS20775 +0.5
BPHYT_RS21600 +0.1
BPHYT_RS22135 -0.2
BPHYT_RS25030 -0.1
BPHYT_RS25415 N.D.
BPHYT_RS25655 +0.1
BPHYT_RS26470 +0.6
BPHYT_RS28250 N.D.
BPHYT_RS30440 +0.0
BPHYT_RS30465 -1.2
BPHYT_RS30795 -0.2
BPHYT_RS31385 +0.3
BPHYT_RS31405 N.D.
BPHYT_RS31610 +0.0
BPHYT_RS31900 -0.2
BPHYT_RS33140 N.D.
BPHYT_RS33705 -0.1
BPHYT_RS34945 N.D.
BPHYT_RS34965 N.D.
BPHYT_RS35505 -0.2
BPHYT_RS35675 -0.2
3-hydroxyoctanoyl-[acyl-carrier-protein] dehydratase:
a (3R,10S)-3-hydroxy-10-methyldodecanoyl-[acp]→a (2E,10S)-10-methyldodec-2-enoyl-[acp] + H2O
(EC 4.2.1.59)
BPHYT_RS12085 N.D.
Enoyl-[acyl-carrier-protein] reductase (NADH) (in reverse):
a (2E,10S)-10-methyldodec-2-enoyl-[acp] + NADH + H+→a (10S)-10-methyldodecanoyl-[acp] + NAD+
(EC 1.3.1.9)
BPHYT_RS01880 +0.0
BPHYT_RS12390 +0.2
BPHYT_RS20615 -0.0
BPHYT_RS30015 N.D.
BPHYT_RS33380 -0.2
Enoyl-[acyl-carrier-protein] reductase (NADPH, B-specific) (in reverse):
a (2E,10S)-10-methyldodec-2-enoyl-[acp] + NADPH + H+→a (10S)-10-methyldodecanoyl-[acp] + NADP+
(EC 1.3.1.10; 1.3.1.104)
BPHYT_RS32935 -0.0
Beta-ketoacyl-acyl-carrier-protein synthase II:
a (10S)-10-methyldodecanoyl-[acp] + a malonyl-[acp] + H+→a (12S)-12-methyl-3-oxotetradecanoyl-[acp] + a soluble [acyl-carrier protein] + CO2
(EC 2.3.1.179)
BPHYT_RS14415 N.D.
BPHYT_RS15130 +0.0
3-oxoacyl-[acyl-carrier-protein] reductase (in reverse):
a (12S)-12-methyl-3-oxotetradecanoyl-[acp] + NADPH + H+→a (3R,12S)-3-hydroxy-12-methyltetradecanoyl-[acp] + NADP+
(EC 1.1.1.100)
BPHYT_RS01050 -0.1
BPHYT_RS04770 +0.0
BPHYT_RS05365 -0.2
BPHYT_RS05385 +0.3
BPHYT_RS06420 N.D.
BPHYT_RS07065 -0.0
BPHYT_RS08285 N.D.
BPHYT_RS08365 -0.0
BPHYT_RS08890 -0.2
BPHYT_RS10170 +0.1
BPHYT_RS10200 N.D.
BPHYT_RS10595 -0.1
BPHYT_RS10630 +0.1
BPHYT_RS10735 N.D.
BPHYT_RS11845 N.D.
BPHYT_RS13055 -0.5
BPHYT_RS14425 N.D.
BPHYT_RS15135 +0.1
BPHYT_RS15890 -1.4
BPHYT_RS16040 N.D.
BPHYT_RS16940 -2.2
BPHYT_RS20510 N.D.
BPHYT_RS20625 +0.1
BPHYT_RS20775 +0.5
BPHYT_RS21600 +0.1
BPHYT_RS22135 -0.2
BPHYT_RS25030 -0.1
BPHYT_RS25415 N.D.
BPHYT_RS25655 +0.1
BPHYT_RS26470 +0.6
BPHYT_RS28250 N.D.
BPHYT_RS30440 +0.0
BPHYT_RS30465 -1.2
BPHYT_RS30795 -0.2
BPHYT_RS31385 +0.3
BPHYT_RS31405 N.D.
BPHYT_RS31610 +0.0
BPHYT_RS31900 -0.2
BPHYT_RS33140 N.D.
BPHYT_RS33705 -0.1
BPHYT_RS34945 N.D.
BPHYT_RS34965 N.D.
BPHYT_RS35505 -0.2
BPHYT_RS35675 -0.2
3-hydroxyoctanoyl-[acyl-carrier-protein] dehydratase:
a (3R,12S)-3-hydroxy-12-methyltetradecanoyl-[acp]→a (2E,12S)-12-methyltetradec-2-enoyl-[acp] + H2O
(EC 4.2.1.59)
BPHYT_RS12085 N.D.
Enoyl-[acyl-carrier-protein] reductase (NADH) (in reverse):
a (2E,12S)-12-methyltetradec-2-enoyl-[acp] + NADH + H+→a (12S)-12-methyltetradecanoyl-[acp] + NAD+
(EC 1.3.1.9)
BPHYT_RS01880 +0.0
BPHYT_RS12390 +0.2
BPHYT_RS20615 -0.0
BPHYT_RS30015 N.D.
BPHYT_RS33380 -0.2
Enoyl-[acyl-carrier-protein] reductase (NADPH, B-specific) (in reverse):
a (2E,12S)-12-methyltetradec-2-enoyl-[acp] + NADPH + H+→a (12S)-12-methyltetradecanoyl-[acp] + NADP+
(EC 1.3.1.10; 1.3.1.104; 1.3.1.39)
BPHYT_RS32935 -0.0
Beta-ketoacyl-acyl-carrier-protein synthase II:
a (12S)-12-methyltetradecanoyl-[acp] + a malonyl-[acp] + H+→a (14S)-14-methyl-3-oxohexadecanoyl-[acp] + a soluble [acyl-carrier protein] + CO2
(EC 2.3.1.179)
BPHYT_RS14415 N.D.
BPHYT_RS15130 +0.0
Oleoyl-[acyl-carrier-protein] hydrolase:
a (12S)-12-methyltetradecanoyl-[acp] + H2O→(12S)-12-methyltetradecanoate + a soluble [acyl-carrier protein] + H+
(EC 3.1.2.14)
No genes
3-oxoacyl-[acyl-carrier-protein] reductase (in reverse):
a (14S)-14-methyl-3-oxohexadecanoyl-[acp] + NADPH + H+→a (3R,14S)-3-hydroxy-14-methylhexadecanoyl-[acp] + NADP+
(EC 1.1.1.100)
BPHYT_RS01050 -0.1
BPHYT_RS04770 +0.0
BPHYT_RS05365 -0.2
BPHYT_RS05385 +0.3
BPHYT_RS06420 N.D.
BPHYT_RS07065 -0.0
BPHYT_RS08285 N.D.
BPHYT_RS08365 -0.0
BPHYT_RS08890 -0.2
BPHYT_RS10170 +0.1
BPHYT_RS10200 N.D.
BPHYT_RS10595 -0.1
BPHYT_RS10630 +0.1
BPHYT_RS10735 N.D.
BPHYT_RS11845 N.D.
BPHYT_RS13055 -0.5
BPHYT_RS14425 N.D.
BPHYT_RS15135 +0.1
BPHYT_RS15890 -1.4
BPHYT_RS16040 N.D.
BPHYT_RS16940 -2.2
BPHYT_RS20510 N.D.
BPHYT_RS20625 +0.1
BPHYT_RS20775 +0.5
BPHYT_RS21600 +0.1
BPHYT_RS22135 -0.2
BPHYT_RS25030 -0.1
BPHYT_RS25415 N.D.
BPHYT_RS25655 +0.1
BPHYT_RS26470 +0.6
BPHYT_RS28250 N.D.
BPHYT_RS30440 +0.0
BPHYT_RS30465 -1.2
BPHYT_RS30795 -0.2
BPHYT_RS31385 +0.3
BPHYT_RS31405 N.D.
BPHYT_RS31610 +0.0
BPHYT_RS31900 -0.2
BPHYT_RS33140 N.D.
BPHYT_RS33705 -0.1
BPHYT_RS34945 N.D.
BPHYT_RS34965 N.D.
BPHYT_RS35505 -0.2
BPHYT_RS35675 -0.2
3-hydroxyoctanoyl-[acyl-carrier-protein] dehydratase:
a (3R,14S)-3-hydroxy-14-methylhexadecanoyl-[acp]→a (2E,14S)-14-methylhexadec-2-enoyl-[acp] + H2O
(EC 4.2.1.59)
BPHYT_RS12085 N.D.
Enoyl-[acyl-carrier-protein] reductase (NADH) (in reverse):
a (2E,14S)-14-methylhexadec-2-enoyl-[acp] + NADH + H+→a (14S)-14-methylhexadecanoyl-[acp] + NAD+
(EC 1.3.1.9)
BPHYT_RS01880 +0.0
BPHYT_RS12390 +0.2
BPHYT_RS20615 -0.0
BPHYT_RS30015 N.D.
BPHYT_RS33380 -0.2
Enoyl-[acyl-carrier-protein] reductase (NADPH, B-specific) (in reverse):
a (2E,14S)-14-methylhexadec-2-enoyl-[acp] + NADPH + H+→a (14S)-14-methylhexadecanoyl-[acp] + NADP+
(EC 1.3.1.10; 1.3.1.104)
BPHYT_RS32935 -0.0
Oleoyl-[acyl-carrier-protein] hydrolase:
a (14S)-14-methylhexadecanoyl-[acp] + H2O→(14S)-14-methylhexadecanoate + a soluble [acyl-carrier protein] + H+
(EC 3.1.2.14)
No genes

Links:

Candidate genes for each reaction are identified from best hits to MetaCyc; by matching EC numbers (which are assigned by TIGRFam, SEED, or best hits to KEGG); or by matching SEED roles to KEGG reactions to MetaCyc reactions. See the "Protein" tab of each gene for more information