MetaCyc Pathway: oleate biosynthesis IV (anaerobic) in Burkholderia phytofirmans PsJN

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Reactions and Genes D-Galactose (C)
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3-hydroxyoctanoyl-[acyl-carrier-protein] dehydratase:
a (3R)-3-hydroxydodecanoyl-[acp]→a (2E)-dodec-2-enoyl-[acp] + H2O (EC 4.2.1.59)
BPHYT_RS12085 N.D.
Trans-2-decenoyl-[acyl-carrier-protein] isomerase:
a (2E)-dodec-2-enoyl-[acp]→a (3Z)-dodec-3-enoyl-[acp] (EC 5.3.3.14)
No genes
Beta-ketoacyl-acyl-carrier-protein synthase II:
a (3Z)-dodec-3-enoyl-[acp] + a malonyl-[acp] + H+→a (5Z)-3-oxo-tetradec-5-enoyl-[acp] + a soluble [acyl-carrier protein] + CO2 (EC 2.3.1.179; 2.3.1.41)
BPHYT_RS14415 N.D.
BPHYT_RS15125 +0.0
BPHYT_RS15130 +0.0
BPHYT_RS15145 +0.1
3-oxoacyl-[acyl-carrier-protein] reductase (in reverse):
a (5Z)-3-oxo-tetradec-5-enoyl-[acp][cytosol] + NADPH[cytosol] + H+[cytosol]→a (3R,5Z)-3-hydroxy-tetradec-5-enoyl-[acp][cytosol] + NADP+[cytosol] (EC 1.1.1.100)
BPHYT_RS01050 -0.1
BPHYT_RS04770 +0.0
BPHYT_RS05365 -0.2
BPHYT_RS05385 +0.3
BPHYT_RS06420 N.D.
BPHYT_RS07065 -0.0
BPHYT_RS08285 N.D.
BPHYT_RS08365 -0.0
BPHYT_RS08890 -0.2
BPHYT_RS10170 +0.1
BPHYT_RS10200 N.D.
BPHYT_RS10595 -0.1
BPHYT_RS10630 +0.1
BPHYT_RS10735 N.D.
BPHYT_RS11845 N.D.
BPHYT_RS13055 -0.5
BPHYT_RS14425 N.D.
BPHYT_RS15135 +0.1
BPHYT_RS15890 -1.4
BPHYT_RS16040 N.D.
BPHYT_RS16940 -2.2
BPHYT_RS20510 N.D.
BPHYT_RS20625 +0.1
BPHYT_RS20775 +0.5
BPHYT_RS21600 +0.1
BPHYT_RS22135 -0.2
BPHYT_RS25030 -0.1
BPHYT_RS25415 N.D.
BPHYT_RS25655 +0.1
BPHYT_RS26470 +0.6
BPHYT_RS28250 N.D.
BPHYT_RS30440 +0.0
BPHYT_RS30465 -1.2
BPHYT_RS30795 -0.2
BPHYT_RS31385 +0.3
BPHYT_RS31405 N.D.
BPHYT_RS31610 +0.0
BPHYT_RS31900 -0.2
BPHYT_RS33140 N.D.
BPHYT_RS33705 -0.1
BPHYT_RS34945 N.D.
BPHYT_RS34965 N.D.
BPHYT_RS35505 -0.2
BPHYT_RS35675 -0.2
3-hydroxyoctanoyl-[acyl-carrier-protein] dehydratase:
a (3R,5Z)-3-hydroxy-tetradec-5-enoyl-[acp][cytosol]→a (2E,5Z)-tetradeca-2,5-dienoyl-[acp][cytosol] + H2O[cytosol] (EC 4.2.1.59)
BPHYT_RS12085 N.D.
Enoyl-[acyl-carrier-protein] reductase (NADH) (in reverse):
a (2E,5Z)-tetradeca-2,5-dienoyl-[acp][cytosol] + NADH[cytosol] + H+[cytosol]→a (5Z)-tetradec-5-enoyl-[acp][cytosol] + NAD+[cytosol] (EC 1.3.1.9)
BPHYT_RS01880 +0.0
BPHYT_RS12390 +0.2
BPHYT_RS20615 -0.0
BPHYT_RS30015 N.D.
BPHYT_RS33380 -0.2
Beta-ketoacyl-acyl-carrier-protein synthase II:
a (5Z)-tetradec-5-enoyl-[acp] + a malonyl-[acp] + H+→a (7Z)-3-oxo-hexadec-7-enoyl-[acp] + a soluble [acyl-carrier protein] + CO2 (EC 2.3.1.179; 2.3.1.41)
BPHYT_RS14415 N.D.
BPHYT_RS15125 +0.0
BPHYT_RS15130 +0.0
BPHYT_RS15145 +0.1
3-oxoacyl-[acyl-carrier-protein] reductase (in reverse):
a (7Z)-3-oxo-hexadec-7-enoyl-[acp][cytosol] + NADPH[cytosol] + H+[cytosol]→a (3R,7Z)-3-hydroxy-hexadec-7-enoyl-[acp][cytosol] + NADP+[cytosol] (EC 1.1.1.100)
BPHYT_RS01050 -0.1
BPHYT_RS04770 +0.0
BPHYT_RS05365 -0.2
BPHYT_RS05385 +0.3
BPHYT_RS06420 N.D.
BPHYT_RS07065 -0.0
BPHYT_RS08285 N.D.
BPHYT_RS08365 -0.0
BPHYT_RS08890 -0.2
BPHYT_RS10170 +0.1
BPHYT_RS10200 N.D.
BPHYT_RS10595 -0.1
BPHYT_RS10630 +0.1
BPHYT_RS10735 N.D.
BPHYT_RS11845 N.D.
BPHYT_RS13055 -0.5
BPHYT_RS14425 N.D.
BPHYT_RS15135 +0.1
BPHYT_RS15890 -1.4
BPHYT_RS16040 N.D.
BPHYT_RS16940 -2.2
BPHYT_RS20510 N.D.
BPHYT_RS20625 +0.1
BPHYT_RS20775 +0.5
BPHYT_RS21600 +0.1
BPHYT_RS22135 -0.2
BPHYT_RS25030 -0.1
BPHYT_RS25415 N.D.
BPHYT_RS25655 +0.1
BPHYT_RS26470 +0.6
BPHYT_RS28250 N.D.
BPHYT_RS30440 +0.0
BPHYT_RS30465 -1.2
BPHYT_RS30795 -0.2
BPHYT_RS31385 +0.3
BPHYT_RS31405 N.D.
BPHYT_RS31610 +0.0
BPHYT_RS31900 -0.2
BPHYT_RS33140 N.D.
BPHYT_RS33705 -0.1
BPHYT_RS34945 N.D.
BPHYT_RS34965 N.D.
BPHYT_RS35505 -0.2
BPHYT_RS35675 -0.2
3-hydroxyoctanoyl-[acyl-carrier-protein] dehydratase:
a (3R,7Z)-3-hydroxy-hexadec-7-enoyl-[acp][cytosol]→a (2E,7Z)-hexadeca-2,7-dienoyl-[acp][cytosol] + H2O[cytosol] (EC 4.2.1.59)
BPHYT_RS12085 N.D.
Enoyl-[acyl-carrier-protein] reductase (NADH) (in reverse):
a (2E,7Z)-hexadeca-2,7-dienoyl-[acp][cytosol] + NADH[cytosol] + H+[cytosol]→a (7Z)-hexadec-7-enoyl-[acp][cytosol] + NAD+[cytosol] (EC 1.3.1.9)
BPHYT_RS01880 +0.0
BPHYT_RS12390 +0.2
BPHYT_RS20615 -0.0
BPHYT_RS30015 N.D.
BPHYT_RS33380 -0.2
Beta-ketoacyl-acyl-carrier-protein synthase II:
a (7Z)-hexadec-7-enoyl-[acp] + a malonyl-[acp] + H+→a (9Z)-3-oxo-octadec-9-enoyl-[acp] + a soluble [acyl-carrier protein] + CO2 (EC 2.3.1.179; 2.3.1.41)
BPHYT_RS14415 N.D.
BPHYT_RS15125 +0.0
BPHYT_RS15130 +0.0
BPHYT_RS15145 +0.1
3-oxoacyl-[acyl-carrier-protein] reductase (in reverse):
a (9Z)-3-oxo-octadec-9-enoyl-[acp][cytosol] + NADPH[cytosol] + H+[cytosol]→a (3R,9Z)-3-hydroxy-octadec-9-enoyl-[acp][cytosol] + NADP+[cytosol] (EC 1.1.1.100)
BPHYT_RS01050 -0.1
BPHYT_RS04770 +0.0
BPHYT_RS05365 -0.2
BPHYT_RS05385 +0.3
BPHYT_RS06420 N.D.
BPHYT_RS07065 -0.0
BPHYT_RS08285 N.D.
BPHYT_RS08365 -0.0
BPHYT_RS08890 -0.2
BPHYT_RS10170 +0.1
BPHYT_RS10200 N.D.
BPHYT_RS10595 -0.1
BPHYT_RS10630 +0.1
BPHYT_RS10735 N.D.
BPHYT_RS11845 N.D.
BPHYT_RS13055 -0.5
BPHYT_RS14425 N.D.
BPHYT_RS15135 +0.1
BPHYT_RS15890 -1.4
BPHYT_RS16040 N.D.
BPHYT_RS16940 -2.2
BPHYT_RS20510 N.D.
BPHYT_RS20625 +0.1
BPHYT_RS20775 +0.5
BPHYT_RS21600 +0.1
BPHYT_RS22135 -0.2
BPHYT_RS25030 -0.1
BPHYT_RS25415 N.D.
BPHYT_RS25655 +0.1
BPHYT_RS26470 +0.6
BPHYT_RS28250 N.D.
BPHYT_RS30440 +0.0
BPHYT_RS30465 -1.2
BPHYT_RS30795 -0.2
BPHYT_RS31385 +0.3
BPHYT_RS31405 N.D.
BPHYT_RS31610 +0.0
BPHYT_RS31900 -0.2
BPHYT_RS33140 N.D.
BPHYT_RS33705 -0.1
BPHYT_RS34945 N.D.
BPHYT_RS34965 N.D.
BPHYT_RS35505 -0.2
BPHYT_RS35675 -0.2
3-hydroxyoctanoyl-[acyl-carrier-protein] dehydratase:
a (3R,9Z)-3-hydroxy-octadec-9-enoyl-[acp][cytosol]→a (2E,9Z)-octadeca-2,9-dienoyl-[acp][cytosol] + H2O[cytosol] (EC 4.2.1.59)
BPHYT_RS12085 N.D.
Enoyl-[acyl-carrier-protein] reductase (NADH) (in reverse):
a (2E,9Z)-octadeca-2,9-dienoyl-[acp] + NADH + H+→an oleoyl-[acp] + NAD+ (EC 1.3.1.9)
BPHYT_RS01880 +0.0
BPHYT_RS12390 +0.2
BPHYT_RS20615 -0.0
BPHYT_RS30015 N.D.
BPHYT_RS33380 -0.2

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Candidate genes for each reaction are identified from best hits to MetaCyc; by matching EC numbers (which are assigned by TIGRFam, SEED, or best hits to KEGG); or by matching SEED roles to KEGG reactions to MetaCyc reactions. See the "Protein" tab of each gene for more information