MetaCyc Pathway: pyruvate fermentation to isobutanol (engineered) in Pseudomonas fluorescens FW300-N2E2

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Reactions and Genes L-Arabinose (C)
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Acetolactate synthase:
2 pyruvate + H+→(S)-2-acetolactate + CO2 (EC 2.2.1.6)
Pf6N2E2_5175 -5.7
Pf6N2E2_5176 -5.7
Pf6N2E2_5475 -0.0
Ketol-acid reductoisomerase (in reverse):
(S)-2-acetolactate + NADPH + H+→(2R)-2,3-dihydroxy-3-methylbutanoate + NADP+ (EC 1.1.1.86)
Pf6N2E2_5177 -5.3
Dihydroxy-acid dehydratase:
(2R)-2,3-dihydroxy-3-methylbutanoate→3-methyl-2-oxobutanoate + H2O (EC 4.2.1.9)
Pf6N2E2_1668 -0.1
Pf6N2E2_4564 -5.1
Pf6N2E2_609 -3.9
Branched-chain-2-oxoacid decarboxylase:
3-methyl-2-oxobutanoate + H+→isobutanal + CO2 (EC 4.1.1.72)
No genes
Alcohol dehydrogenase (in reverse):
isobutanal + NADH + H+→isobutanol + NAD+ (EC 1.1.1.1)
Pf6N2E2_1380 N.D.
Pf6N2E2_1522 +0.2
Pf6N2E2_194 +0.3
Pf6N2E2_2802 -0.1
Pf6N2E2_3044 +0.1
Pf6N2E2_4819 -0.1
Pf6N2E2_5980 +0.2
Pf6N2E2_840 -0.3
Pf6N2E2_999 +0.1

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Candidate genes for each reaction are identified from best hits to MetaCyc; by matching EC numbers (which are assigned by TIGRFam, SEED, or best hits to KEGG); or by matching SEED roles to KEGG reactions to MetaCyc reactions. See the "Protein" tab of each gene for more information