MetaCyc Pathway: pyruvate fermentation to isobutanol (engineered) in Sinorhizobium meliloti 1021

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Reactions and Genes L-Arabinose (C)
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Acetolactate synthase:
2 pyruvate + H+→(S)-2-acetolactate + CO2 (EC 2.2.1.6)
SM_b20400 -0.3
SMa0958 -0.0
SMa2211 +0.1
SMc01430 -2.5
SMc01431 -7.3
SMc02263 +0.0
SMc04455 +0.1
Ketol-acid reductoisomerase (in reverse):
(S)-2-acetolactate + NADPH + H+→(2R)-2,3-dihydroxy-3-methylbutanoate + NADP+ (EC 1.1.1.86)
SMc04346 -3.6
Dihydroxy-acid dehydratase:
(2R)-2,3-dihydroxy-3-methylbutanoate→3-methyl-2-oxobutanoate + H2O (EC 4.2.1.9)
SM_b20115 +0.1
SM_b20890 -5.5
SMa0235 +0.1
SMc00884 +0.1
SMc04045 -7.1
SMc04144 -0.3
Branched-chain-2-oxoacid decarboxylase:
3-methyl-2-oxobutanoate + H+→isobutanal + CO2 (EC 4.1.1.72)
No genes
Alcohol dehydrogenase (in reverse):
isobutanal + NADH + H+→isobutanol + NAD+ (EC 1.1.1.1)
SM_b20147 -0.2
SM_b20170 N.D.
SMa0263 +0.1
SMa1296 +0.2
SMa2113 +0.4
SMc00105 +0.3
SMc00680 +0.2
SMc01270 N.D.
SMc01582 +0.0
SMc01816 -0.1
SMc03929 +0.1
SMc04270 N.D.

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Candidate genes for each reaction are identified from best hits to MetaCyc; by matching EC numbers (which are assigned by TIGRFam, SEED, or best hits to KEGG); or by matching SEED roles to KEGG reactions to MetaCyc reactions. See the "Protein" tab of each gene for more information