MetaCyc Pathway: L-isoleucine biosynthesis II in Sinorhizobium meliloti 1021

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Reactions and Genes L-Arabinose (C)
remove
pyruvate + acetyl-CoA + H2O→(R)-citramalate + coenzyme A + H+ (EC 2.3.3.21)
No genes
(R)-2-methylmalate dehydratase:
(R)-citramalate→citraconate + H2O (EC 4.2.1.35)
SMc03795 -4.2
SMc03823 -7.5
citraconate + H2O→(2R,3S)-3-methylmalate
SMc03795 -4.2
SMc03823 -7.5
(2R,3S)-3-methylmalate + NAD+→2-oxobutanoate + CO2 + NADH
No genes
Acetolactate synthase:
2-oxobutanoate + pyruvate + H+→(S)-2-aceto-2-hydroxybutanoate + CO2 (EC 2.2.1.6)
SM_b20400 -0.3
SMa0958 -0.0
SMa2211 +0.1
SMc01430 -2.5
SMc01431 -7.3
SMc02263 +0.0
SMc04455 +0.1
(S)-2-aceto-2-hydroxybutanoate + NAD(P)H + H+→(R)-2,3-dihydroxy-3-methylpentanoate + NAD(P)+ (EC 1.1.1.383)
No genes
Dihydroxy-acid dehydratase:
(R)-2,3-dihydroxy-3-methylpentanoate→(3S)-3-methyl-2-oxopentanoate + H2O (EC 4.2.1.9)
SM_b20115 +0.1
SM_b20890 -5.5
SMa0235 +0.1
SMc00884 +0.1
SMc04045 -7.1
SMc04144 -0.3
Branched-chain-amino-acid transaminase (in reverse):
(3S)-3-methyl-2-oxopentanoate + L-glutamate→L-isoleucine + 2-oxoglutarate (EC 2.6.1.42)
SMc00042 +0.1
SMc02896 +0.2

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Candidate genes for each reaction are identified from best hits to MetaCyc; by matching EC numbers (which are assigned by TIGRFam, SEED, or best hits to KEGG); or by matching SEED roles to KEGG reactions to MetaCyc reactions. See the "Protein" tab of each gene for more information