MetaCyc Pathway: pyruvate fermentation to isobutanol (engineered) in Burkholderia phytofirmans PsJN

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Reactions and Genes L-Arabinose (C)
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Acetolactate synthase:
2 pyruvate + H+→(S)-2-acetolactate + CO2 (EC 2.2.1.6)
BPHYT_RS06145 -0.1
BPHYT_RS06545 -4.2
BPHYT_RS06550 +0.5
BPHYT_RS10500 -0.1
BPHYT_RS19345 +0.0
BPHYT_RS31395 +0.4
BPHYT_RS34970 +0.1
Ketol-acid reductoisomerase (in reverse):
(S)-2-acetolactate + NADPH + H+→(2R)-2,3-dihydroxy-3-methylbutanoate + NADP+ (EC 1.1.1.86)
BPHYT_RS06555 -3.7
Dihydroxy-acid dehydratase:
(2R)-2,3-dihydroxy-3-methylbutanoate→3-methyl-2-oxobutanoate + H2O (EC 4.2.1.9)
BPHYT_RS04815 +0.2
BPHYT_RS10100 -1.9
BPHYT_RS19730 -2.8
BPHYT_RS21270 -0.1
BPHYT_RS25650 -0.4
BPHYT_RS31695 -0.1
Branched-chain-2-oxoacid decarboxylase:
3-methyl-2-oxobutanoate + H+→isobutanal + CO2 (EC 4.1.1.72)
No genes
Alcohol dehydrogenase (in reverse):
isobutanal + NADH + H+→isobutanol + NAD+ (EC 1.1.1.1)
BPHYT_RS00510 +0.0
BPHYT_RS16150 -0.1
BPHYT_RS20205 -0.2
BPHYT_RS24470 -0.3
BPHYT_RS25430 N.D.
BPHYT_RS27390 -0.1
BPHYT_RS28135 +0.2
BPHYT_RS30075 -0.2
BPHYT_RS31305 -0.7
BPHYT_RS31860 N.D.

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Candidate genes for each reaction are identified from best hits to MetaCyc; by matching EC numbers (which are assigned by TIGRFam, SEED, or best hits to KEGG); or by matching SEED roles to KEGG reactions to MetaCyc reactions. See the "Protein" tab of each gene for more information