MetaCyc Pathway: (R)- and (S)-3-hydroxybutanoate biosynthesis (engineered) in Marinobacter adhaerens HP15

Add experiment(s):


Reactions and Genes L-Phenylalanine as (N)
remove
Acetyl-CoA C-acyltransferase:
2 acetyl-CoA→acetoacetyl-CoA + coenzyme A (EC 2.3.1.16; 2.3.1.9)
HP15_1009 +0.2
HP15_1233 +0.1
HP15_1513 -0.4
HP15_2344 +0.2
HP15_2423 -0.1
HP15_2695 -3.0
HP15_2996 -1.0
HP15_35 -0.2
HP15_3943 -0.1
HP15_49 +0.1
HP15_5 +0.3
3-hydroxyacyl-CoA dehydrogenase (in reverse):
acetoacetyl-CoA + NADH + H+→(S)-3-hydroxybutanoyl-CoA + NAD+ (EC 1.1.1.35)
HP15_1232 -0.3
HP15_1512 -0.2
HP15_3941 -0.4
HP15_48 +0.3
Acetoacetyl-CoA reductase (in reverse):
acetoacetyl-CoA + NADPH + H+→(3R)-3-hydroxybutanoyl-CoA + NADP+ (EC 1.1.1.36)
HP15_2784 -0.3
(S)-3-hydroxybutanoyl-CoA + H2O→(S)-3-hydroxybutanoate + coenzyme A + H+
HP15_3348 -0.1
(3R)-3-hydroxybutanoyl-CoA + H2O→(R)-3-hydroxybutanoate + coenzyme A + H+
HP15_3348 -0.1

Links:

Candidate genes for each reaction are identified from best hits to MetaCyc; by matching EC numbers (which are assigned by TIGRFam, SEED, or best hits to KEGG); or by matching SEED roles to KEGG reactions to MetaCyc reactions. See the "Protein" tab of each gene for more information