MetaCyc Pathway: L-isoleucine biosynthesis II in Marinobacter adhaerens HP15

Reactions and Genes	L-Isoleucine as (N)
pyruvate + acetyl-CoA + H2O→(R)-citramalate + coenzyme A + H+ (EC 2.3.3.21)
No genes
(R)-2-methylmalate dehydratase: (R)-citramalate→citraconate + H2O (EC 4.2.1.35)
HP15_1833	-3.6
HP15_1834	-2.0
HP15_2598	+0.1
HP15_2599	-0.1
citraconate + H2O→(2R,3S)-3-methylmalate
HP15_1834	-2.0
(2R,3S)-3-methylmalate + NAD+→2-oxobutanoate + CO2 + NADH
No genes
Acetolactate synthase: 2-oxobutanoate + pyruvate + H+→(S)-2-aceto-2-hydroxybutanoate + CO2 (EC 2.2.1.6)
HP15_1016	+0.2
HP15_1017	-1.0
HP15_3118	-0.0
HP15_531	-1.8
HP15_532	-1.7
HP15_953	+0.0
(S)-2-aceto-2-hydroxybutanoate + NAD(P)H + H+→(R)-2,3-dihydroxy-3-methylpentanoate + NAD(P)+ (EC 1.1.1.383)
No genes
Dihydroxy-acid dehydratase: (R)-2,3-dihydroxy-3-methylpentanoate→(3S)-3-methyl-2-oxopentanoate + H2O (EC 4.2.1.9)
HP15_3363	-0.0
HP15_595	+0.0
Branched-chain-amino-acid transaminase (in reverse): (3S)-3-methyl-2-oxopentanoate + L-glutamate→L-isoleucine + 2-oxoglutarate (EC 2.6.1.42)
HP15_436	N.D.

Candidate genes for each reaction are identified from best hits to MetaCyc; by matching EC numbers (which are assigned by TIGRFam, SEED, or best hits to KEGG); or by matching SEED roles to KEGG reactions to MetaCyc reactions. See the "Protein" tab of each gene for more information