MetaCyc Pathway: superpathway of glyoxylate cycle and fatty acid degradation in Shewanella amazonensis SB2B

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Reactions and Genes Tween 20
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a 2,3,4-saturated fatty acid + ATP + coenzyme A→a 2,3,4-saturated fatty acyl CoA + AMP + diphosphate
No genes
Acyl-CoA oxidase:
a 2,3,4-saturated fatty acyl CoA + dioxygen→a (2E)-2-enoyl-CoA + hydrogen peroxide
(EC 1.3.3.6)
No genes
Enoyl-CoA hydratase (in reverse):
a (2E)-2-enoyl-CoA + H2O→a 2,3,4-saturated (3S)-3-hydroxyacyl-CoA
(EC 4.2.1.17)
Sama_0032 (fadB) -5.1
Sama_1378 +0.3
Sama_1379 -2.5
Sama_2167 (fadJ) +0.0
Sama_3052 +0.2
Sama_3135 +0.1
3-hydroxyacyl-CoA dehydrogenase:
a 2,3,4-saturated (3S)-3-hydroxyacyl-CoA + NAD+→a 2,3,4-saturated 3-oxoacyl-CoA + NADH + H+
(EC 1.1.1.35)
Sama_0032 (fadB) -5.1
Sama_2167 (fadJ) +0.0
Acetyl-CoA C-acyltransferase (in reverse):
a 2,3,4-saturated 3-oxoacyl-CoA + coenzyme A→a 2,3,4-saturated fatty acyl CoA + acetyl-CoA
(EC 2.3.1.16)
Sama_0031 (fadA) -4.4
Sama_1375 +0.2
Sama_2168 (fadI) +0.0
Citrate (Si)-synthase:
acetyl-CoA + oxaloacetate + H2O→citrate + coenzyme A + H+
(EC 2.3.3.1; 2.3.3.16; 2.3.3.3)
Sama_1422 (gltA) -3.7
Aconitate hydratase:
citrate→cis-aconitate + H2O
(EC 4.2.1.3)
Sama_0382 N.D.
Sama_3296 -1.5
Aconitate hydratase:
cis-aconitate + H2O→D-threo-isocitrate
(EC 4.2.1.3)
Sama_0382 N.D.
Sama_3296 -1.5
Isocitrate lyase:
D-threo-isocitrate→glyoxylate + succinate
(EC 4.1.3.1)
Sama_2380 -4.3
Malate synthase:
acetyl-CoA + glyoxylate + H2O→(S)-malate + coenzyme A + H+
(EC 2.3.3.9)
Sama_0681 -2.3
Sama_2381 -4.2
Succinate dehydrogenase (ubiquinone):
succinate[in] + an electron-transfer quinone→fumarate[in] + an electron-transfer quinol
(EC 1.3.5.1)
No genes
Fumarate hydratase (in reverse):
fumarate + H2O→(S)-malate
(EC 4.2.1.2)
Sama_0822 -0.0
Sama_1688 N.D.
Malate dehydrogenase:
(S)-malate + NAD+→oxaloacetate + NADH + H+
(EC 1.1.1.37; 1.1.1.38)
Sama_0672 -3.9
Sama_2621 -3.0
Phosphoenolpyruvate carboxykinase (ATP):
oxaloacetate + ATP→phosphoenolpyruvate + ADP + CO2
(EC 4.1.1.49)
Sama_0165 -0.9

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Candidate genes for each reaction are identified from best hits to MetaCyc; by matching EC numbers (which are assigned by TIGRFam, SEED, or best hits to KEGG); or by matching SEED roles to KEGG reactions to MetaCyc reactions. See the "Protein" tab of each gene for more information