MetaCyc Pathway: glutathione-mediated detoxification I in Pseudomonas fluorescens SBW25-INTG

Reactions and Genes	Glycine betaine (C)(N); with MOPS
Glutathione transferase: RX + glutathione→a glutathione-S-conjugate + HX (EC 2.5.1.18)
PFLU_RS05065	+0.6
PFLU_RS05095	+0.1
PFLU_RS06500	+0.0
PFLU_RS10550	+0.0
PFLU_RS11005	+0.1
PFLU_RS11675	+0.8
PFLU_RS11695	-0.1
PFLU_RS16675	-0.1
PFLU_RS17375	+0.1
PFLU_RS18500	-0.1
PFLU_RS20890	+0.2
PFLU_RS21350	-0.0
PFLU_RS23545	+0.0
PFLU_RS23610	+0.3
PFLU_RS23645	-0.0
PFLU_RS29225	+0.2
a glutathione-S-conjugate[in] + ATP[in] + H2O[in]→a glutathione-S-conjugate[out] + ADP[in] + H+[in] + phosphate[in] (EC 7.6.2.3)
No genes
a glutathione-S-conjugate + H2O→an (L-cysteinylglycine)-S-conjugate + L-glutamate (EC 3.4.19.13)
No genes
an (L-cysteinylglycine)-S-conjugate + H2O→an L-cysteine-S-conjugate + glycine (EC 3.4.13.23)
No genes
Cysteine-S-conjugate N-acetyltransferase: an L-cysteine-S-conjugate + acetyl-CoA→a mercapturate + coenzyme A + H+ (EC 2.3.1.80)
No genes
Cysteine-S-conjugate beta-lyase: an L-cysteine-S-conjugate→2-aminoprop-2-enoate + a thiol (EC 4.4.1.13)
No genes
Tryptophanase: 2-aminoprop-2-enoate→2-iminopropanoate (spontaneous) (EC 4.1.99.1; 4.1.99.2; 4.3.1.13; 4.3.1.17; 4.3.1.18; 4.3.3.8; 4.4.1.1; 4.4.1.13; 4.4.1.15; 4.4.1.25; 4.4.1.28; 4.4.1.35; 4.5.1.2)
PFLU_RS01220	+0.3
PFLU_RS05135	+0.5
PFLU_RS12815	+0.0
PFLU_RS16625	+0.1
PFLU_RS24005	+0.3
PFLU_RS27960	-1.2
2-iminopropanoate + H2O→pyruvate + ammonium (spontaneous) (EC 3.5.99.10; 4.1.99.1; 4.1.99.2; 4.3.1.13; 4.3.1.17; 4.3.1.18; 4.3.3.8; 4.4.1.1; 4.4.1.13; 4.4.1.15; 4.4.1.25; 4.4.1.28; 4.4.1.35; 4.5.1.2)
PFLU_RS01220	+0.3
PFLU_RS05135	+0.5
PFLU_RS12815	+0.0
PFLU_RS16625	+0.1
PFLU_RS24005	+0.3
PFLU_RS27960	-1.2
PFLU_RS29515	-2.4

Candidate genes for each reaction are identified from best hits to MetaCyc; by matching EC numbers (which are assigned by TIGRFam, SEED, or best hits to KEGG); or by matching SEED roles to KEGG reactions to MetaCyc reactions. See the "Protein" tab of each gene for more information