MetaCyc Pathway: glycerol degradation to butanol in Pseudomonas fluorescens SBW25-INTG

Add experiment(s):


Reactions and Genes L-Glutamine (C)(N); with MOPS; with chloride
remove
Glycerol dehydrogenase:
glycerol + NAD+→dihydroxyacetone + NADH + H+ (EC 1.1.1.6)
No genes
Glycerone kinase:
dihydroxyacetone + ATP→glycerone phosphate + ADP + H+ (EC 2.7.1.29)
No genes
Triose-phosphate isomerase (in reverse):
glycerone phosphate→D-glyceraldehyde 3-phosphate (EC 5.3.1.1)
PFLU_RS25840 N.D.
Glyceraldehyde-3-phosphate dehydrogenase (phosphorylating):
D-glyceraldehyde 3-phosphate + NAD+ + phosphate→3-phospho-D-glyceroyl phosphate + NADH + H+ (EC 1.2.1.12)
PFLU_RS07715 N.D.
PFLU_RS24335 -0.2
Phosphoglycerate kinase (in reverse):
3-phospho-D-glyceroyl phosphate + ADP→3-phospho-D-glycerate + ATP (EC 2.7.2.3)
PFLU_RS28085 N.D.
3-phospho-D-glycerate→2-phospho-D-glycerate (EC 5.4.2.12)
PFLU_RS01655 N.D.
Phosphopyruvate hydratase:
2-phospho-D-glycerate→phosphoenolpyruvate + H2O (EC 4.2.1.11)
PFLU_RS06375 N.D.
PFLU_RS09395 +0.1
Pyruvate kinase (in reverse):
phosphoenolpyruvate + ADP + H+→pyruvate + ATP (EC 2.7.1.40)
PFLU_RS08850 -0.0
PFLU_RS24255 -0.0
Pyruvate synthase:
pyruvate + coenzyme A + 2 an oxidized ferredoxin [iron-sulfur] cluster→acetyl-CoA + CO2 + H+ + 2 a reduced ferredoxin [iron-sulfur] cluster (EC 1.2.7.1)
No genes
Acetyl-CoA C-acyltransferase:
2 acetyl-CoA→acetoacetyl-CoA + coenzyme A (EC 2.3.1.16; 2.3.1.9)
PFLU_RS03225 -0.3
PFLU_RS06735 +0.0
PFLU_RS07655 -0.9
PFLU_RS10575 -0.2
PFLU_RS14765 -0.1
PFLU_RS16065 +0.6
PFLU_RS17190 +0.4
PFLU_RS21180 +0.0
PFLU_RS22845 -0.2
3-hydroxyacyl-CoA dehydrogenase (in reverse):
acetoacetyl-CoA + NADH + H+→(S)-3-hydroxybutanoyl-CoA + NAD+ (EC 1.1.1.35)
PFLU_RS07650 -1.0
PFLU_RS11090 -0.2
PFLU_RS22840 +0.0
(S)-3-hydroxybutanoyl-CoA→crotonyl-CoA + H2O (EC 4.2.1.150)
PFLU_RS07105 -0.3
PFLU_RS07650 -1.0
PFLU_RS08485 +0.5
PFLU_RS10835 +0.0
PFLU_RS12025 -1.4
PFLU_RS24260 +0.1
crotonyl-CoA[cytosol] + 2 NADH[cytosol] + 2 an oxidized ferredoxin [iron-sulfur] cluster[cytosol]→butanoyl-CoA[cytosol] + 2 NAD+[cytosol] + 2 a reduced ferredoxin [iron-sulfur] cluster[cytosol] (EC 1.3.1.109)
No genes
Butanal dehydrogenase (in reverse):
butanoyl-CoA + NAD(P)H + H+→1-butanal + coenzyme A + NAD(P)+ (EC 1.2.1.57)
No genes
Alcohol dehydrogenase (NADP(+)) (in reverse):
1-butanal + NADPH + H+→butan-1-ol + NADP+ (EC 1.1.1.2)
PFLU_RS13450 +0.2
1-butanal + NADH + H+→butan-1-ol + NAD+
No genes

Links:

Candidate genes for each reaction are identified from best hits to MetaCyc; by matching EC numbers (which are assigned by TIGRFam, SEED, or best hits to KEGG); or by matching SEED roles to KEGG reactions to MetaCyc reactions. See the "Protein" tab of each gene for more information