MetaCyc Pathway: pyruvate fermentation to butanol II (engineered) in Burkholderia phytofirmans PsJN

Reactions and Genes	Parafilmed volatile agar plate with Trichoderma atroviridae IMI
Acetyl-CoA C-acyltransferase: 2 acetyl-CoA→acetoacetyl-CoA + coenzyme A (EC 2.3.1.16; 2.3.1.9)
BPHYT_RS02230	+0.3
BPHYT_RS03230	+0.1
BPHYT_RS08275	-0.1
BPHYT_RS09150	+0.2
BPHYT_RS09180	+0.1
BPHYT_RS09565	+2.2
BPHYT_RS17345	+0.4
BPHYT_RS21955	+0.5
BPHYT_RS29385	-0.1
3-hydroxyacyl-CoA dehydrogenase (in reverse): acetoacetyl-CoA + NADH + H+→(S)-3-hydroxybutanoyl-CoA + NAD+ (EC 1.1.1.35)
BPHYT_RS03225	+0.1
BPHYT_RS06760	-0.2
BPHYT_RS09560	-0.6
BPHYT_RS13545	-0.3
BPHYT_RS28020	N.D.
BPHYT_RS34155	+0.3
(S)-3-hydroxybutanoyl-CoA→crotonyl-CoA + H2O (EC 4.2.1.150)
BPHYT_RS02345	+0.6
BPHYT_RS03225	+0.1
BPHYT_RS03235	-0.1
BPHYT_RS03825	N.D.
BPHYT_RS08375	N.D.
BPHYT_RS08630	-0.2
BPHYT_RS09560	-0.6
BPHYT_RS10815	N.D.
BPHYT_RS11780	N.D.
BPHYT_RS13545	-0.3
BPHYT_RS14830	-0.2
BPHYT_RS25360	N.D.
BPHYT_RS25695	N.D.
BPHYT_RS27105	+0.1
BPHYT_RS27895	-0.2
BPHYT_RS28620	+0.6
BPHYT_RS35465	-0.4
Trans-2-enoyl-CoA reductase (NAD(+)) (in reverse): crotonyl-CoA + NADH + H+→butanoyl-CoA + NAD+ (EC 1.3.1.44)
BPHYT_RS00670	+0.3
BPHYT_RS02220	-0.3
BPHYT_RS03000	-0.2
BPHYT_RS08290	+0.1
BPHYT_RS08510	+0.1
BPHYT_RS20325	+0.2
BPHYT_RS20780	-0.3
BPHYT_RS25370	+0.2
BPHYT_RS25400	+2.8
BPHYT_RS28040	-0.3
Butanal dehydrogenase (in reverse): butanoyl-CoA + NAD(P)H + H+→1-butanal + coenzyme A + NAD(P)+ (EC 1.2.1.57)
No genes
1-butanal + NADH + H+→butan-1-ol + NAD+
No genes

Candidate genes for each reaction are identified from best hits to MetaCyc; by matching EC numbers (which are assigned by TIGRFam, SEED, or best hits to KEGG); or by matching SEED roles to KEGG reactions to MetaCyc reactions. See the "Protein" tab of each gene for more information