MetaCyc Pathway: mixed acid fermentation in Klebsiella michiganensis M5al

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Reactions and Genes D-Glucose (C) and no Nitrogen; normal Wolfe's minerals with nitroloacetic; 8 days
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Phosphoenolpyruvate carboxylase (in reverse):
phosphoenolpyruvate + hydrogen carbonate→oxaloacetate + phosphate (EC 4.1.1.31)
BWI76_RS00835 -1.3
Pyruvate kinase (in reverse):
phosphoenolpyruvate + ADP + H+→pyruvate + ATP (EC 2.7.1.40)
BWI76_RS16960 N.D.
BWI76_RS18115 +0.3
D-lactate dehydrogenase (in reverse):
pyruvate + NADH + H+→(R)-lactate + NAD+ (EC 1.1.1.28)
BWI76_RS12790 +0.3
BWI76_RS19515 -0.4
Malate dehydrogenase (in reverse):
oxaloacetate + NADH + H+→(S)-malate + NAD+ (EC 1.1.1.37; 1.1.1.38)
BWI76_RS13830 -0.1
BWI76_RS16775 -0.5
BWI76_RS25420 +0.4
Formate C-acetyltransferase (in reverse):
pyruvate + coenzyme A→acetyl-CoA + formate (EC 2.3.1.54)
BWI76_RS03570 -0.0
BWI76_RS03575 +0.0
BWI76_RS09070 +0.3
BWI76_RS09075 +0.1
BWI76_RS09730 -0.0
BWI76_RS09735 -1.0
BWI76_RS10655 +0.1
BWI76_RS10660 +0.0
BWI76_RS21375 -0.1
Acetaldehyde dehydrogenase (acetylating) (in reverse):
acetyl-CoA + NADH + H+→acetaldehyde + coenzyme A + NAD+ (EC 1.2.1.10)
BWI76_RS02835 -0.1
BWI76_RS03590 -0.1
BWI76_RS16905 +0.2
BWI76_RS17250 -1.5
BWI76_RS20790 -0.1
Citrate (Si)-synthase:
acetyl-CoA + oxaloacetate + H2O→citrate + coenzyme A + H+ (EC 2.3.3.1; 2.3.3.16; 2.3.3.3)
BWI76_RS08385 -1.4
BWI76_RS19140 -0.2
formate + H+→CO2 + H2
BWI76_RS02115 -0.0
BWI76_RS16780 +0.1
BWI76_RS17560 +0.1
BWI76_RS22325 -0.6
BWI76_RS22330 -0.5
BWI76_RS22335 -0.3
Fumarate hydratase:
(S)-malate→fumarate + H2O (EC 4.2.1.2)
BWI76_RS08450 -0.0
BWI76_RS16115 +0.2
BWI76_RS16270 -0.1
BWI76_RS16275 -0.5
BWI76_RS16955 -0.7
Phosphate acetyltransferase:
acetyl-CoA + phosphate→acetyl phosphate + coenzyme A (EC 2.3.1.8)
BWI76_RS03560 -0.0
BWI76_RS20240 N.D.
BWI76_RS20805 -0.4
BWI76_RS23150 +0.0
Acetate kinase (in reverse):
acetyl phosphate + ADP→acetate + ATP (EC 2.7.2.1; 2.7.2.15)
BWI76_RS18090 +0.1
BWI76_RS20235 N.D.
BWI76_RS20815 +0.1
BWI76_RS20820 +0.0
BWI76_RS23200 -0.2
Aconitate hydratase:
citrate→cis-aconitate + H2O (EC 4.2.1.3)
BWI76_RS04910 N.D.
BWI76_RS11940 +0.1
Alcohol dehydrogenase (in reverse):
acetaldehyde + NADH + H+→ethanol + NAD+ (EC 1.1.1.1)
BWI76_RS00645 +0.2
BWI76_RS02835 -0.1
BWI76_RS03140 +0.2
BWI76_RS03320 +0.1
BWI76_RS12395 -0.2
BWI76_RS13800 -0.0
BWI76_RS15240 -0.1
BWI76_RS17250 -1.5
BWI76_RS25510 -0.1
BWI76_RS27960 -0.1
Succinate dehydrogenase (ubiquinone):
fumarate[in] + a menaquinol→succinate[in] + a menaquinone (EC 1.3.5.1)
BWI76_RS02440 -0.7
BWI76_RS02445 -0.5
BWI76_RS02450 -1.0
BWI76_RS02455 -0.8
Aconitate hydratase:
cis-aconitate + H2O→D-threo-isocitrate (EC 4.2.1.3)
BWI76_RS04910 N.D.
BWI76_RS11940 +0.1
Isocitrate dehydrogenase (NADP(+)):
D-threo-isocitrate + NADP+→CO2 + 2-oxoglutarate + NADPH (EC 1.1.1.42)
BWI76_RS11330 N.D.

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Candidate genes for each reaction are identified from best hits to MetaCyc; by matching EC numbers (which are assigned by TIGRFam, SEED, or best hits to KEGG); or by matching SEED roles to KEGG reactions to MetaCyc reactions. See the "Protein" tab of each gene for more information