MetaCyc Pathway: (S)-lactate fermentation to propanoate, acetate and hydrogen in Klebsiella michiganensis M5al

Add experiment(s):


Reactions and Genes D-Glucose (C) with ammonium chloride; anaerobic; 8 days
remove
L-lactate dehydrogenase:
(S)-lactate + NAD+→pyruvate + NADH + H+ (EC 1.1.1.27)
BWI76_RS15220 +0.0
Pyruvate carboxylase:
pyruvate + ATP + hydrogen carbonate→oxaloacetate + ADP + H+ + phosphate (EC 6.4.1.1)
No genes
Formate C-acetyltransferase (in reverse):
pyruvate + coenzyme A→acetyl-CoA + formate (EC 2.3.1.54)
BWI76_RS03570 -0.0
BWI76_RS03575 -0.1
BWI76_RS09070 +0.1
BWI76_RS09075 +0.2
BWI76_RS09730 -2.6
BWI76_RS09735 -2.9
BWI76_RS10655 +0.2
BWI76_RS10660 +0.0
BWI76_RS21375 -0.3
formate + H+→H2 + CO2
BWI76_RS02115 -0.2
BWI76_RS16780 +0.1
BWI76_RS17560 -0.0
BWI76_RS22325 -0.8
BWI76_RS22330 -0.5
BWI76_RS22335 -0.4
Malate dehydrogenase (in reverse):
oxaloacetate + NADH + H+→(S)-malate + NAD+ (EC 1.1.1.37; 1.1.1.38)
BWI76_RS13830 +0.2
BWI76_RS16775 -0.1
BWI76_RS25420 +0.4
Phosphate acetyltransferase:
acetyl-CoA + phosphate→acetyl phosphate + coenzyme A (EC 2.3.1.8)
BWI76_RS03560 +0.1
BWI76_RS20240 N.D.
BWI76_RS20805 -0.1
BWI76_RS23150 +0.2
Acetate kinase (in reverse):
acetyl phosphate + ADP→acetate + ATP (EC 2.7.2.1; 2.7.2.15)
BWI76_RS18090 +0.1
BWI76_RS20235 N.D.
BWI76_RS20815 +0.0
BWI76_RS20820 +0.1
BWI76_RS23200 +0.0
Fumarate hydratase:
(S)-malate→fumarate + H2O (EC 4.2.1.2)
BWI76_RS08450 +0.1
BWI76_RS16115 +0.3
BWI76_RS16270 +0.0
BWI76_RS16275 -0.5
BWI76_RS16955 -0.6
Succinate dehydrogenase (ubiquinone):
fumarate[in] + a menaquinol→succinate[in] + a menaquinone (EC 1.3.5.1)
BWI76_RS02440 -1.5
BWI76_RS02445 -1.5
BWI76_RS02450 -1.5
BWI76_RS02455 -1.7
succinate + propanoyl-CoA→succinyl-CoA + propanoate (EC 2.8.3.27)
BWI76_RS23950 -0.0
Methylmalonyl-CoA mutase (in reverse):
succinyl-CoA→(R)-methylmalonyl-CoA (EC 5.4.99.2)
BWI76_RS23935 +0.2
Methylmalonyl-CoA epimerase:
(R)-methylmalonyl-CoA→(S)-methylmalonyl-CoA (EC 5.1.99.1)
No genes
(S)-methylmalonyl-CoA[in] + Na+[in] + H+[out]→propanoyl-CoA[in] + CO2[in] + Na+[out] (EC 7.2.4.3)
No genes

Links:

Candidate genes for each reaction are identified from best hits to MetaCyc; by matching EC numbers (which are assigned by TIGRFam, SEED, or best hits to KEGG); or by matching SEED roles to KEGG reactions to MetaCyc reactions. See the "Protein" tab of each gene for more information