MetaCyc Pathway: camalexin biosynthesis in Pseudomonas stutzeri RCH2

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Reactions and Genes 1-ethyl-3-methylimidazolium acetate 40 mM
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L-tryptophan + dioxygen + a reduced [NADPH-hemoprotein reductase]→N2-hydroxy-L-tryptophan + an oxidized [NADPH-hemoprotein reductase] + H+ + H2O (EC 1.14.14.156)
No genes
N2-hydroxy-L-tryptophan + dioxygen + a reduced [NADPH-hemoprotein reductase]→N2,N2-dihydroxy-L-tryptophan + an oxidized [NADPH-hemoprotein reductase] + H2O (EC 1.14.14.156)
No genes
N2,N2-dihydroxy-L-tryptophan + H+→(E)-(indol-3-yl)acetaldehyde oxime + CO2 + H2O (spontaneous) (EC 1.14.14.156)
No genes
(E)-(indol-3-yl)acetaldehyde oxime→(Z)-indol-3-ylacetaldoxime (EC 1.14.14.M36; 1.14.14.M40)
No genes
(Z)-indol-3-ylacetaldoxime→(indol-3-yl)acetonitrile + H2O (EC 1.14.14.M36; 1.14.14.M40)
No genes
(indol-3-yl)acetonitrile + dioxygen + a reduced [NADPH-hemoprotein reductase]→2-hydroxy-2-(1H-indol-3-yl)acetonitrile + an oxidized [NADPH-hemoprotein reductase] + H2O (EC 1.14.14.M36; 1.14.14.M40)
No genes
2-hydroxy-2-(1H-indol-3-yl)acetonitrile→dehydro(indole-3-yl)acetonitrile + H2O (EC 1.14.14.M36)
No genes
Glutathione transferase:
dehydro(indole-3-yl)acetonitrile + glutathione→(glutathion-S-yl)(1H-indol-3-yl)acetonitrile (EC 2.5.1.18)
Psest_1131 -0.3
Psest_1219 +0.2
Psest_1421 -0.6
Psest_1859 -0.2
Psest_1991 -3.5
Psest_2378 -0.1
Psest_2571 -0.0
Psest_3511 -0.2
Psest_0049 -0.1
Psest_0056 -0.1
Psest_0868 -0.2
(glutathion-S-yl)(1H-indol-3-yl)acetonitrile + H2O→(L-cysteinylglycin-S-yl)(1H-indol-3-yl)acetonitrile + L-glutamate (EC 3.4.19.16)
No genes
(L-cysteinylglycin-S-yl)(1H-indol-3-yl)acetonitrile + H2O→2-(cystein-S-yl)-2-(1H-indol-3-yl)-acetonitrile + glycine (EC 3.4.13.23)
No genes
2-(cystein-S-yl)-2-(1H-indol-3-yl)-acetonitrile + dioxygen + a reduced [NADPH-hemoprotein reductase]→(R)-dihydrocamalexate + hydrogen cyanide + an oxidized [NADPH-hemoprotein reductase] + H+ + 2 H2O (EC 1.14.19.52)
No genes
(R)-dihydrocamalexate + dioxygen + H+ + a reduced [NADPH-hemoprotein reductase]→camalexin + CO2 + an oxidized [NADPH-hemoprotein reductase] + 2 H2O (EC 1.14.19.52)
No genes

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Candidate genes for each reaction are identified from best hits to MetaCyc; by matching EC numbers (which are assigned by TIGRFam, SEED, or best hits to KEGG); or by matching SEED roles to KEGG reactions to MetaCyc reactions. See the "Protein" tab of each gene for more information