MetaCyc Pathway: indole glucosinolate activation (intact plant cell) in Pseudomonas stutzeri RCH2

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Reactions and Genes acetate 80 mM
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Thioglucosidase:
glucobrassicin + H2O→(indol-3-yl)-N-(sulfonatooxy)methanimidothioate + D-glucopyranose + H+ (EC 3.2.1.147)
No genes
glucobrassicin + dioxygen + a reduced [NADPH-hemoprotein reductase]→4-hydroxyglucobrassicin + an oxidized [NADPH-hemoprotein reductase] + H2O (EC 1.14.13.M51)
No genes
4-hydroxyglucobrassicin + S-adenosyl-L-methionine→4-methoxyglucobrassicin + S-adenosyl-L-homocysteine + H+
No genes
(indol-3-yl)-N-(sulfonatooxy)methanimidothioate→indolylmethylisothiocyanate + sulfate (spontaneous)
No genes
Thioglucosidase:
4-methoxyglucobrassicin + H2O→4-methoxy-3-indolylmethyl glucosinolate aglycone + D-glucopyranose + H+ (EC 3.2.1.147)
No genes
Glutathione transferase:
indolylmethylisothiocyanate + glutathione→indol-3-ylmethylisothiocyanate-glutathione (EC 2.5.1.18)
Psest_1131 +0.2
Psest_1219 +0.0
Psest_1421 +0.2
Psest_1859 -0.2
Psest_1991 -1.3
Psest_2378 +0.1
Psest_2571 +0.1
Psest_3511 +0.1
Psest_0049 -0.1
Psest_0056 +0.1
Psest_0868 +0.0
indol-3-ylmethylisothiocyanate-glutathione + H2O→indol-3-ylmethylisothiocyanate-[γ-Glu-Cys} + glycine (EC 3.4.17.25)
No genes
4-methoxy-3-indolylmethyl glucosinolate aglycone→4-methoxy-3-indolylmethylisothiocyanate + sulfate (spontaneous)
No genes
indol-3-ylmethylisothiocyanate-[γ-Glu-Cys} + H2O→indol-3-ylmethylisothiocyanate-L-cysteine + L-glutamate (EC 3.4.19.13)
No genes
4-methoxy-3-indolylmethylisothiocyanate + glutathione→(glutathion-S-yl)-4-methoxy-3-indolylmethylisothiocyanate
No genes
(glutathion-S-yl)-4-methoxy-3-indolylmethylisothiocyanate + 2 H2O→raphanusamate + 4-methoxy-3-indolylmethylamine + L-glutamate + glycine
No genes
indol-3-ylmethylisothiocyanate-L-cysteine→raphanusamate + 3-(aminomethyl)indole
No genes

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Candidate genes for each reaction are identified from best hits to MetaCyc; by matching EC numbers (which are assigned by TIGRFam, SEED, or best hits to KEGG); or by matching SEED roles to KEGG reactions to MetaCyc reactions. See the "Protein" tab of each gene for more information