MetaCyc Pathway: L-leucine degradation I in Pseudomonas fluorescens SBW25-INTG

Add experiment(s):


Reactions and Genes 4-Hydroxybenzoic Acid (C) and Ammonium chloride (N); with MOPS
remove
Branched-chain-amino-acid transaminase:
L-leucine + 2-oxoglutarate→4-methyl-2-oxopentanoate + L-glutamate (EC 2.6.1.42; 2.6.1.6)
PFLU_RS19375 -5.1
2-oxoisovalerate dehydrogenase (acylating):
4-methyl-2-oxopentanoate + coenzyme A + NAD+→3-methylbutanoyl-CoA + CO2 + NADH (EC 1.2.1.25)
No genes
3-methylbutanoyl-CoA + an oxidized electron-transfer flavoprotein + H+→3-methylcrotonyl-CoA + a reduced electron-transfer flavoprotein (EC 1.3.8.4)
PFLU_RS18940 -3.0
PFLU_RS18965 -1.5
Methylcrotonoyl-CoA carboxylase:
3-methylcrotonyl-CoA + ATP + hydrogen carbonate→3-methylglutaconyl-CoA + ADP + H+ + phosphate (EC 6.4.1.4)
PFLU_RS18970 -1.3
PFLU_RS18980 -1.8
Methylglutaconyl-CoA hydratase (in reverse):
3-methylglutaconyl-CoA + H2O→(S)-3-hydroxy-3-methylglutaryl-CoA (EC 4.2.1.18)
PFLU_RS18975 -1.3
Hydroxymethylglutaryl-CoA lyase:
(S)-3-hydroxy-3-methylglutaryl-CoA→acetoacetate + acetyl-CoA (EC 4.1.3.4)
PFLU_RS06990 +0.2
PFLU_RS18955 -1.0

Links:

Candidate genes for each reaction are identified from best hits to MetaCyc; by matching EC numbers (which are assigned by TIGRFam, SEED, or best hits to KEGG); or by matching SEED roles to KEGG reactions to MetaCyc reactions. See the "Protein" tab of each gene for more information