MetaCyc Pathway: superpathway of glycolysis and the Entner-Doudoroff pathway in Pseudomonas fluorescens FW300-N2E2

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Reactions and Genes Nitrate reduction in presence of wild-type Pseudomonas N2-E3; replicate C
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Glucose-6-phosphate dehydrogenase:
D-glucopyranose 6-phosphate + NADP+→6-phospho D-glucono-1,5-lactone + NADPH + H+ (EC 1.1.1.49)
Pf6N2E2_2885 +0.1
Pf6N2E2_758 +0.2
D-glucopyranose[out] + an [HPr protein]-Nπ-phospho-L-histidine[in]→D-glucopyranose 6-phosphate[in] + an [HPr]-L-histidine[in] (EC 2.7.1.199)
No genes
6-phosphogluconolactonase:
6-phospho D-glucono-1,5-lactone + H2O→D-gluconate 6-phosphate + H+ (EC 3.1.1.31)
Pf6N2E2_1924 +0.1
Pf6N2E2_2884 N.D.
Glucose-6-phosphate isomerase:
α-D-glucose 6-phosphate→β-D-fructofuranose 6-phosphate (EC 5.3.1.9)
Pf6N2E2_5150 +1.3
6-phosphofructokinase:
β-D-fructofuranose 6-phosphate + ATP→β-D-fructofuranose 1,6-bisphosphate + ADP + H+ (EC 2.7.1.11)
No genes
Phosphogluconate dehydratase:
D-gluconate 6-phosphate→2-dehydro-3-deoxy-D-gluconate 6-phosphate + H2O (EC 4.2.1.12)
Pf6N2E2_2898 N.D.
Fructose-bisphosphate aldolase:
β-D-fructofuranose 1,6-bisphosphate→glycerone phosphate + D-glyceraldehyde 3-phosphate (EC 4.1.2.13)
Pf6N2E2_4660 N.D.
Fructose-bisphosphatase:
β-D-fructofuranose 1,6-bisphosphate + H2O→β-D-fructofuranose 6-phosphate + phosphate (EC 3.1.3.11)
Pf6N2E2_3813 N.D.
2-dehydro-3-deoxy-phosphogluconate aldolase:
2-dehydro-3-deoxy-D-gluconate 6-phosphate→D-glyceraldehyde 3-phosphate + pyruvate (EC 4.1.2.14; 4.1.2.55)
Pf6N2E2_2883 N.D.
Glyceraldehyde-3-phosphate dehydrogenase (phosphorylating):
D-glyceraldehyde 3-phosphate + NAD+ + phosphate→3-phospho-D-glyceroyl phosphate + NADH + H+ (EC 1.2.1.12)
Pf6N2E2_2277 -4.1
Pf6N2E2_2899 +0.1
Triose-phosphate isomerase (in reverse):
glycerone phosphate→D-glyceraldehyde 3-phosphate (EC 5.3.1.1)
Pf6N2E2_3356 N.D.
Phosphoglycerate kinase (in reverse):
3-phospho-D-glyceroyl phosphate + ADP→3-phospho-D-glycerate + ATP (EC 2.7.2.3)
Pf6N2E2_4657 N.D.
3-phospho-D-glycerate→2-phospho-D-glycerate (EC 5.4.2.12)
Pf6N2E2_3832 N.D.
3-phospho-D-glycerate→2-phospho-D-glycerate (EC 5.4.2.11)
No genes
Phosphopyruvate hydratase:
2-phospho-D-glycerate→phosphoenolpyruvate + H2O (EC 4.2.1.11)
Pf6N2E2_3048 N.D.
Pyruvate kinase (in reverse):
phosphoenolpyruvate + ADP + H+→pyruvate + ATP (EC 2.7.1.40)
Pf6N2E2_5494 +0.2
Pf6N2E2_5835 +0.2
Pyruvate, water dikinase:
pyruvate + ATP + H2O→phosphoenolpyruvate + AMP + 2 H+ + phosphate (EC 2.7.9.2)
Pf6N2E2_2389 -1.8

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Candidate genes for each reaction are identified from best hits to MetaCyc; by matching EC numbers (which are assigned by TIGRFam, SEED, or best hits to KEGG); or by matching SEED roles to KEGG reactions to MetaCyc reactions. See the "Protein" tab of each gene for more information