MetaCyc Pathway: queuosine biosynthesis I (de novo) in Pseudomonas fluorescens SBW25-INTG

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Reactions and Genes p-Coumaric (C) and Ammonium chloride (N); with MOPS
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PreQ(1) synthase (in reverse):
preQ0 + 2 NADPH + 3 H+→preQ1 + 2 NADP+ (EC 1.7.1.13)
PFLU_RS07770 +1.4
tRNA-guanine transglycosylase:
preQ1 + a guanine34 in tRNA→a 7-aminomethyl-7-deazaguanosine34 in tRNA + guanine (EC 2.4.2.29)
PFLU_RS24900 +3.0
a 7-aminomethyl-7-deazaguanosine34 in tRNA + S-adenosyl-L-methionine→an epoxyqueuosine34 in tRNA + adenine + L-methionine + H+ (EC 2.4.99.17)
PFLU_RS24905 +2.6
an epoxyqueuosine34 in tRNA + a reduced two electron carrier→a queuosine34 in tRNA + an oxidized electron carrier + H2O (EC 1.17.99.6)
PFLU_RS02540 -0.6

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Candidate genes for each reaction are identified from best hits to MetaCyc; by matching EC numbers (which are assigned by TIGRFam, SEED, or best hits to KEGG); or by matching SEED roles to KEGG reactions to MetaCyc reactions. See the "Protein" tab of each gene for more information