MetaCyc Pathway: L-asparagine biosynthesis III (tRNA-dependent) in Pseudomonas fluorescens SBW25-INTG

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Reactions and Genes Betaine (C)(N); with MOPS; with chloride
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Glutamate synthase (NADPH):
L-glutamine + H2O→ammonia + L-glutamate + H+ (EC 1.4.1.13; 1.4.1.14; 1.4.7.1; 2.6.1.85; 3.5.1.2; 3.5.1.38; 4.1.3.27; 4.3.2.10; 4.3.3.6; 6.3.4.2; 6.3.5.13; 6.3.5.2; 6.3.5.4; 6.3.5.5; 6.3.5.6; 6.3.5.7)
PFLU_RS02045 -4.1
PFLU_RS02050 -4.3
PFLU_RS04290 N.D.
PFLU_RS04295 N.D.
PFLU_RS04300 N.D.
PFLU_RS05320 -1.0
PFLU_RS05490 +0.1
PFLU_RS06365 N.D.
PFLU_RS06610 -0.2
PFLU_RS06710 -0.3
PFLU_RS06830 +0.0
PFLU_RS06835 +0.2
PFLU_RS08375 +0.1
PFLU_RS08710 +0.1
PFLU_RS11415 -0.2
PFLU_RS11420 -0.1
PFLU_RS12015 +0.3
PFLU_RS15070 +0.1
PFLU_RS16015 -0.3
PFLU_RS17490 -0.1
PFLU_RS19210 +0.2
PFLU_RS19255 -0.2
PFLU_RS19480 +0.6
PFLU_RS20385 -0.0
PFLU_RS21200 +0.0
PFLU_RS21955 +0.6
PFLU_RS22715 -2.5
PFLU_RS22835 -3.2
PFLU_RS23455 -0.5
PFLU_RS23780 -0.8
PFLU_RS24590 -4.4
PFLU_RS24730 N.D.
PFLU_RS25880 N.D.
PFLU_RS25885 N.D.
PFLU_RS26915 +0.2
PFLU_RS27375 -1.6
PFLU_RS27380 -1.4
PFLU_RS30020 +0.1
Aspartate--tRNA(Asn) ligase:
L-aspartate + tRNAAsn + ATP→an L-aspartyl-[tRNAAsn] + AMP + diphosphate (EC 6.1.1.23)
PFLU_RS24105 N.D.
Asparaginyl-tRNA synthase (glutamine-hydrolyzing):
an L-aspartyl-[tRNAAsn] + ATP→a 4-phosphooxy-L-aspartyl-[tRNAAsn] + ADP (EC 6.3.5.6)
PFLU_RS04290 N.D.
PFLU_RS04295 N.D.
PFLU_RS04300 N.D.
PFLU_RS05320 -1.0
PFLU_RS19480 +0.6
Asparaginyl-tRNA synthase (glutamine-hydrolyzing):
a 4-phosphooxy-L-aspartyl-[tRNAAsn] + ammonia→an L-asparaginyl-[tRNAAsn] + phosphate (EC 6.3.5.6)
PFLU_RS04290 N.D.
PFLU_RS04295 N.D.
PFLU_RS04300 N.D.
PFLU_RS05320 -1.0
PFLU_RS19480 +0.6

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Candidate genes for each reaction are identified from best hits to MetaCyc; by matching EC numbers (which are assigned by TIGRFam, SEED, or best hits to KEGG); or by matching SEED roles to KEGG reactions to MetaCyc reactions. See the "Protein" tab of each gene for more information