MetaCyc Pathway: superpathway of L-isoleucine biosynthesis I in Ralstonia sp. UNC404CL21Col

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Reactions and Genes D-Xylose (C)
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Aspartate transaminase (in reverse):
oxaloacetate + L-glutamate→L-aspartate + 2-oxoglutarate (EC 2.6.1.1)
ABZR87_RS04630 +0.1
ABZR87_RS05850 +0.0
ABZR87_RS05990 +0.2
ABZR87_RS08250 +0.1
ABZR87_RS08830 +0.1
ABZR87_RS08860 -0.0
ABZR87_RS09835 +0.2
ABZR87_RS11440 -2.7
ABZR87_RS14645 +0.0
ABZR87_RS17005 +0.0
ABZR87_RS18535 +0.0
ABZR87_RS19655 +0.1
ABZR87_RS21785 -0.1
Aspartate kinase:
L-aspartate + ATP→L-aspartyl-4-phosphate + ADP (EC 2.7.2.4)
ABZR87_RS10665 N.D.
Aspartate-semialdehyde dehydrogenase (in reverse):
L-aspartyl-4-phosphate + NADPH + H+→L-aspartate 4-semialdehyde + NADP+ + phosphate (EC 1.2.1.11)
ABZR87_RS14520 N.D.
Homoserine dehydrogenase (in reverse):
L-aspartate 4-semialdehyde + NAD(P)H + H+→L-homoserine + NAD(P)+ (EC 1.1.1.3)
ABZR87_RS11445 N.D.
Homoserine kinase:
L-homoserine + ATP→O-phospho-L-homoserine + ADP + H+ (EC 2.7.1.39)
ABZR87_RS05860 +0.1
ABZR87_RS15695 N.D.
Threonine synthase:
O-phospho-L-homoserine + H2O→L-threonine + phosphate (EC 4.2.3.1)
ABZR87_RS05865 -0.0
ABZR87_RS11450 N.D.
Threonine ammonia-lyase:
L-threonine→(Z)-2-aminobutenoate + H2O (EC 4.3.1.19)
ABZR87_RS07360 -1.8
ABZR87_RS09675 +0.1
ABZR87_RS17670 +0.1
1-aminocyclopropane-1-carboxylate deaminase:
(Z)-2-aminobutenoate→2-iminobutanoate (spontaneous) (EC 3.5.99.7; 4.3.1.19; 4.4.1.1; 4.4.1.11; 4.4.1.2)
ABZR87_RS07360 -1.8
ABZR87_RS09675 +0.1
ABZR87_RS13980 -0.0
ABZR87_RS17670 +0.1
2-iminobutanoate + H2O→2-oxobutanoate + ammonium (spontaneous) (EC 3.5.99.10; 3.5.99.7; 4.3.1.19; 4.4.1.1; 4.4.1.11; 4.4.1.2)
ABZR87_RS07360 -1.8
ABZR87_RS09675 +0.1
ABZR87_RS13980 -0.0
ABZR87_RS17670 +0.1
Acetolactate synthase:
2-oxobutanoate + H+ + pyruvate→(S)-2-aceto-2-hydroxybutanoate + CO2 (EC 2.2.1.6)
ABZR87_RS11575 +0.0
ABZR87_RS14950 -1.9
ABZR87_RS14955 -2.7
ABZR87_RS20655 +0.0
Ketol-acid reductoisomerase (in reverse):
(S)-2-aceto-2-hydroxybutanoate + NADPH + H+→(R)-2,3-dihydroxy-3-methylpentanoate + NADP+ (EC 1.1.1.86)
ABZR87_RS14945 N.D.
Dihydroxy-acid dehydratase:
(R)-2,3-dihydroxy-3-methylpentanoate→(3S)-3-methyl-2-oxopentanoate + H2O (EC 4.2.1.9)
ABZR87_RS00135 +0.2
ABZR87_RS00240 +0.0
ABZR87_RS18260 -2.6
Branched-chain-amino-acid transaminase (in reverse):
(3S)-3-methyl-2-oxopentanoate + L-glutamate→L-isoleucine + 2-oxoglutarate (EC 2.6.1.42)
ABZR87_RS18540 +0.0

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Candidate genes for each reaction are identified from best hits to MetaCyc; by matching EC numbers (which are assigned by TIGRFam, SEED, or best hits to KEGG); or by matching SEED roles to KEGG reactions to MetaCyc reactions. See the "Protein" tab of each gene for more information