MetaCyc Pathway: superpathway of L-methionine biosynthesis (transsulfuration) in Pseudomonas fluorescens FW300-N2E3

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Reactions and Genes L-Ornithine
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Aspartate transaminase (in reverse):
oxaloacetate + L-glutamate→L-aspartate + 2-oxoglutarate (EC 2.6.1.1)
AO353_01120 -0.1
AO353_01210 -2.4
AO353_01655 -0.1
AO353_02295 +0.4
AO353_09180 +0.0
AO353_09990 +0.5
AO353_15915 -0.4
AO353_17700 -0.1
AO353_18575 +0.7
AO353_19150 +0.1
AO353_20995 -0.0
AO353_22840 -0.2
AO353_22920 +2.2
AO353_23080 +0.0
AO353_24850 +0.4
AO353_26890 +0.1
AO353_28150 +0.7
AO353_29325 -0.7
Aspartate kinase:
L-aspartate + ATP→L-aspartyl-4-phosphate + ADP (EC 2.7.2.4)
AO353_02975 +1.0
Aspartate-semialdehyde dehydrogenase (in reverse):
L-aspartyl-4-phosphate + NADPH + H+→L-aspartate 4-semialdehyde + NADP+ + phosphate (EC 1.2.1.11)
AO353_17160 +0.2
AO353_20640 N.D.
AO353_20645 -0.8
Homoserine dehydrogenase (in reverse):
L-aspartate 4-semialdehyde + NAD(P)H + H+→L-homoserine + NAD(P)+ (EC 1.1.1.3)
AO353_04355 -0.3
Homoserine O-succinyltransferase:
L-homoserine + succinyl-CoA→O-succinyl-L-homoserine + coenzyme A (EC 2.3.1.46)
No genes
Cystathionine gamma-synthase:
O-succinyl-L-homoserine + L-cysteine→L-cystathionine + H+ + succinate (EC 2.5.1.48)
AO353_20695 -3.1
Cysteine-S-conjugate beta-lyase:
L-cystathionine + H2O→L-homocysteine + ammonium + pyruvate (EC 4.4.1.13)
No genes
5-methyltetrahydropteroyltriglutamate--homocysteine S-methyltransferase:
L-homocysteine + 5-methyltetrahydropteroyl tri-L-glutamate→L-methionine + tetrahydropteroyl tri-L-glutamate (EC 2.1.1.14)
AO353_16215 -0.3
Methionine synthase:
L-homocysteine + a 5-methyltetrahydrofolate→L-methionine + a tetrahydrofolate (EC 2.1.1.13)
AO353_21615 -0.8

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Candidate genes for each reaction are identified from best hits to MetaCyc; by matching EC numbers (which are assigned by TIGRFam, SEED, or best hits to KEGG); or by matching SEED roles to KEGG reactions to MetaCyc reactions. See the "Protein" tab of each gene for more information