MetaCyc Pathway: superpathway of L-isoleucine biosynthesis I in Pseudomonas fluorescens FW300-N2E3

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Reactions and Genes L-Ornithine
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Aspartate transaminase (in reverse):
oxaloacetate + L-glutamate→L-aspartate + 2-oxoglutarate (EC 2.6.1.1)
AO353_01120 -0.1
AO353_01210 -2.4
AO353_01655 -0.1
AO353_02295 +0.4
AO353_09180 +0.0
AO353_09990 +0.5
AO353_15915 -0.4
AO353_17700 -0.1
AO353_18575 +0.7
AO353_19150 +0.1
AO353_20995 -0.0
AO353_22840 -0.2
AO353_22920 +2.2
AO353_23080 +0.0
AO353_24850 +0.4
AO353_26890 +0.1
AO353_28150 +0.7
AO353_29325 -0.7
Aspartate kinase:
L-aspartate + ATP→L-aspartyl-4-phosphate + ADP (EC 2.7.2.4)
AO353_02975 +1.0
Aspartate-semialdehyde dehydrogenase (in reverse):
L-aspartyl-4-phosphate + NADPH + H+→L-aspartate 4-semialdehyde + NADP+ + phosphate (EC 1.2.1.11)
AO353_17160 +0.2
AO353_20640 N.D.
AO353_20645 -0.8
Homoserine dehydrogenase (in reverse):
L-aspartate 4-semialdehyde + NAD(P)H + H+→L-homoserine + NAD(P)+ (EC 1.1.1.3)
AO353_04355 -0.3
Homoserine kinase:
L-homoserine + ATP→O-phospho-L-homoserine + ADP + H+ (EC 2.7.1.39)
AO353_00930 +0.2
AO353_10920 N.D.
Threonine synthase:
O-phospho-L-homoserine + H2O→L-threonine + phosphate (EC 4.2.3.1)
AO353_04350 N.D.
Threonine ammonia-lyase:
L-threonine→(Z)-2-aminobutenoate + H2O (EC 4.3.1.19)
AO353_08485 -1.0
AO353_25960 -0.3
AO353_28645 +0.0
1-aminocyclopropane-1-carboxylate deaminase:
(Z)-2-aminobutenoate→2-iminobutanoate (spontaneous) (EC 3.5.99.7; 4.3.1.19; 4.4.1.1; 4.4.1.11; 4.4.1.2)
AO353_08485 -1.0
AO353_20225 -0.5
AO353_23295 -0.0
AO353_25960 -0.3
AO353_28645 +0.0
2-iminobutanoate + H2O→2-oxobutanoate + ammonium (spontaneous) (EC 3.5.99.10; 3.5.99.7; 4.3.1.19; 4.4.1.1; 4.4.1.11; 4.4.1.2)
AO353_08485 -1.0
AO353_09285 -0.1
AO353_20225 -0.5
AO353_23295 -0.0
AO353_24440 -0.3
AO353_25960 -0.3
AO353_28645 +0.0
Acetolactate synthase:
2-oxobutanoate + H+ + pyruvate→(S)-2-aceto-2-hydroxybutanoate + CO2 (EC 2.2.1.6)
AO353_03865 +0.3
AO353_14495 -1.2
AO353_14500 -2.1
AO353_23145 -0.4
AO353_24845 +0.5
Ketol-acid reductoisomerase (in reverse):
(S)-2-aceto-2-hydroxybutanoate + NADPH + H+→(R)-2,3-dihydroxy-3-methylpentanoate + NADP+ (EC 1.1.1.86)
AO353_14505 -2.9
Dihydroxy-acid dehydratase:
(R)-2,3-dihydroxy-3-methylpentanoate→(3S)-3-methyl-2-oxopentanoate + H2O (EC 4.2.1.9)
AO353_08345 -2.5
Branched-chain-amino-acid transaminase (in reverse):
(3S)-3-methyl-2-oxopentanoate + L-glutamate→L-isoleucine + 2-oxoglutarate (EC 2.6.1.42)
AO353_26655 +0.2

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Candidate genes for each reaction are identified from best hits to MetaCyc; by matching EC numbers (which are assigned by TIGRFam, SEED, or best hits to KEGG); or by matching SEED roles to KEGG reactions to MetaCyc reactions. See the "Protein" tab of each gene for more information