MetaCyc Pathway: ceramide degradation by α-oxidation in Pseudomonas fluorescens SBW25-INTG

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Reactions and Genes D,L-Malic Acid (C) and Ammonium chloride (N); with TAPS; with chloride
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a phytoceramide + 2 a ferrocytochrome b5 + dioxygen + 2 H+→a (2'R)-2'-hydroxy-phytoceramide + 2 a ferricytochrome b5 + H2O (EC 1.14.18.6)
No genes
Ceramidase:
a (2'R)-2'-hydroxy-phytoceramide + H2O→a (2R)-2-hydroxy even numbered straight chain 2,3,4-saturated fatty acid + a phytosphingosine
(EC 3.5.1.23)
No genes
a (2R)-2-hydroxy even numbered straight chain 2,3,4-saturated fatty acid + ATP + coenzyme A→a (R)-2-hydroxy even numbered straight chain 2,3,4-saturated fatty acyl CoA + AMP + diphosphate
No genes
a (R)-2-hydroxy even numbered straight chain 2,3,4-saturated fatty acyl CoA→formyl-CoA + an odd numbered straight chain 2,3,4-saturated fatty aldehyde (EC 4.1.2.63)
No genes
Formyl-CoA hydrolase:
formyl-CoA + H2O→formate + coenzyme A + H+
(EC 3.1.2.10)
No genes
Aldehyde dehydrogenase (NAD(+)):
an odd numbered straight chain 2,3,4-saturated fatty aldehyde + NAD+ + H2O→an odd numbered straight chain 2,3,4-saturated fatty acid + NADH + 2 H+
(EC 1.2.1.3)
PFLU_RS02715 -0.1
PFLU_RS04240 -0.2
PFLU_RS07400 -0.4
PFLU_RS09895 -0.7
PFLU_RS11535 -0.3
PFLU_RS15055 -0.1
PFLU_RS15075 +0.1
PFLU_RS15110 +0.3
PFLU_RS19825 -0.8
PFLU_RS20100 -0.0
PFLU_RS26745 -1.5
PFLU_RS29420 -1.5
Long-chain-fatty-acid--CoA ligase:
an odd numbered straight chain 2,3,4-saturated fatty acid + ATP + coenzyme A→an odd numbered straight chain 2,3,4-saturated fatty acyl CoA + AMP + diphosphate
(EC 6.2.1.3)
PFLU_RS17185 -0.1
PFLU_RS21255 -0.0
PFLU_RS23680 -1.5
PFLU_RS23685 -0.5

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Candidate genes for each reaction are identified from best hits to MetaCyc; by matching EC numbers (which are assigned by TIGRFam, SEED, or best hits to KEGG); or by matching SEED roles to KEGG reactions to MetaCyc reactions. See the "Protein" tab of each gene for more information