MetaCyc Pathway: TCA cycle VII (acetate-producers) in Pseudomonas fluorescens SBW25-INTG

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Reactions and Genes D,L-Malic Acid (C) and Ammonium chloride (N); with TAPS; with chloride
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2-oxoglutarate synthase:
2-oxoglutarate + coenzyme A + 2 an oxidized ferredoxin [iron-sulfur] cluster→succinyl-CoA + CO2 + H+ + 2 a reduced ferredoxin [iron-sulfur] cluster (EC 1.2.7.3)
No genes
succinyl-CoA + acetate→acetyl-CoA + succinate (EC 2.8.3.18)
No genes
Succinate dehydrogenase (ubiquinone):
succinate[in] + an electron-transfer quinone→fumarate[in] + an electron-transfer quinol (EC 1.3.5.1)
PFLU_RS08935 N.D.
PFLU_RS08940 N.D.
PFLU_RS08945 N.D.
PFLU_RS08950 N.D.
Fumarate hydratase (in reverse):
fumarate + H2O→(S)-malate (EC 4.2.1.2)
PFLU_RS04360 -0.8
PFLU_RS21170 -0.1
PFLU_RS24270 N.D.
(S)-malate[in] + an electron-transfer quinone→oxaloacetate[in] + an electron-transfer quinol (EC 1.1.5.4)
PFLU_RS04510 N.D.
PFLU_RS07940 -3.5
Citrate (Si)-synthase:
acetyl-CoA + oxaloacetate + H2O→citrate + coenzyme A + H+ (EC 2.3.3.1; 2.3.3.16; 2.3.3.3)
PFLU_RS08930 N.D.
Aconitate hydratase:
citrate→cis-aconitate + H2O (EC 4.2.1.3)
PFLU_RS07595 +0.1
PFLU_RS17020 N.D.
PFLU_RS22680 -0.1
Aconitate hydratase:
cis-aconitate + H2O→D-threo-isocitrate (EC 4.2.1.3)
PFLU_RS07595 +0.1
PFLU_RS17020 N.D.
PFLU_RS22680 -0.1
Isocitrate dehydrogenase (NADP(+)):
D-threo-isocitrate + NADP+→2-oxoglutarate + CO2 + NADPH (EC 1.1.1.42)
PFLU_RS18565 -0.1
PFLU_RS18570 N.D.

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Candidate genes for each reaction are identified from best hits to MetaCyc; by matching EC numbers (which are assigned by TIGRFam, SEED, or best hits to KEGG); or by matching SEED roles to KEGG reactions to MetaCyc reactions. See the "Protein" tab of each gene for more information